摘要
目的建立HPLC法同时测定银黄颗粒中绿原酸、木犀草苷和黄芩苷的方法。方法采用Kromasil C18柱(250 mm×4.6 mm,5μm),乙腈-0.4%磷酸水溶液为流动相的梯度洗脱模式分离各测定组分,采用可变波长的方式测定相应组分的峰面积并计算样品含量。结果绿原酸、木犀草苷和黄芩苷分别在0.020 13～2.013μg(r=0.999 9)、0.004 93～0.493μg(r=0.999 8)及0.057 62～5.762 43μg(r=0.999 9)范围内具有良好的线性关系;且平均加样回收率分别为100.2%,RSD为0.2%(n=6);99.1%,RSD为2.0%(n=6);100.8%,RSD为1.4%(n=6);重复性试验,3个成分含量的RSD均小于2.0%(n=6)。结论所建立的方法简便、快速、准确,具有良好的重复性和稳定性,可用于银黄颗粒的质量控制。
Objective To establish an HPLC method of simultaneously determining the content of chlorogenic acid,luteolin-7-O-glucoside and Baicalin in Yinghuang granules.Methods Kromasil C18 column（250 mm×4.6 mm,5 μm） was employed with both a gradient elution mode and a variable detecting wavelength in order to determine the concentration of the components simultaneously.Results Chlorogenic acid,luteolin-7-O-glucoside and Baicalin all had good linearity in the ranges of 0.020 13~2.013 μg（r=0.999 9）,0.004 93~0.493 μg（r=0.999 8）,0.057 62~5.762 43 μg（r=0.999 9）,respectively.The average recoveries were 100.2%（RSD=0.2%,n=6）,99.1%（RSD=2.0%,n=6）,and 100.8%（RSD=1.4%,n=6）,correspondingly.Repeatability experiments presented that RSD values of their contents were less than 2.0%.Conclusion It is a simple,rapid and accurate method with high repeatability and stability,and can be used to control the qualities of Yinghuang granules.
出处
《今日药学》
CAS
2013年第8期490-493,共4页
Pharmacy Today
关键词
银黄颗粒
绿原酸
木犀草苷
黄芩苷
高效液相色谱
Yinhuang granules
chlorogenic acid
luteolin-7-O-glucoside
baicalin
HPLC
