摘要
目的:建立高效液相色谱(HPLC)测定参松养心胶囊中人参皂苷Rb1含量的方法,为参松养心胶囊的质量标准控制提供理论依据。方法:样品经甲醇回流提取2 h,水饱和正丁醇萃取,氨试液洗涤。采用Agilent 1200RRLC高分离度快速液相色谱仪,waters XTERRAMS C18(4.6 mm×250 mm,5μm)色谱柱,柱温室温,流动相乙腈-水(53∶47),流速1.0 mL.min-1,检测波长203 nm,进样体积40μL。结果:人参皂苷Rb1进样量在5.0～200.0 mg.L-1与峰面积呈良好线性关系(r=0.998 9);平均回收率为100.31%,RSD 1.98%。结论:实验所建立的方法专属性强,准确度高,重复性好,可作为参松养心胶囊质量控制。
Objective： To establish a method for measuring the content of Ginsenoside Rb1 in Shensong Yangxin capsules by HPLC, in order to provide theoretical basis for the quality standards of Shensong Yangxin capsules. Method： The Ginsenoside Rb1 from Shensong Yangxin capsules was extracted by methanol reflux extraction 2 h, and then extracted by water-saturated n-butanol with, ammonia solution washing. The content of Ginsenoside Rb1 in Shensong Yangxin capsules was measured by HPLC, the instrument and chromatographic conditions was Agilenill00 high-performance liquid chromatograph, waters XTERRA MS C18 （4.6 mm × 250 mm, 5 μm）, column temperature ： room temperature ; mobile phase ： acetonitrile-water （53 ： 47） ; flow rate 1.0 mL·min^-1 ; detection wavelength 203 nm; injection volume 40 μL. Result： The Ginsenoside Rb1 within the range of 5.0-200,0 mg· L^-1 was linear with the peak area, r 0.998 9; the average recovery was 100.31%, RSD 1.98%. Conclusion： The established method has strong specificity, high accuracy and good reproducibility, it can be used as quality control of Shensong Yangxin capsules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第12期112-114,共3页
Chinese Journal of Experimental Traditional Medical Formulae
