摘要
目的:探讨人巨细胞病毒(human cytomegalovirus,HCMV)感染在结直肠癌发生、发展过程中的作用及其可能的作用机制。方法:选择84例结直肠腺癌患者的腺癌组织及自身癌旁正常组织,应用RT-PCR技术检测组织中即刻早期基因2(immediate-earlygene 2,IE2)mRNA的阳性率来反映HCMV的感染情况,免疫组化技术检测凋亡相关基因C-Jun的表达情况。结果:HCMV IE2基因在结直肠腺癌组织中的阳性率高于癌旁组织(P<0.01);C-Jun基因在结直肠腺癌组织中表达的阳性率高于癌旁组织(P<0.01);HCMV感染与C-Jun基因在结直肠腺癌组织内的表达存在关联性。结论:HCMV感染在结直肠癌的发生、发展中具有一定作用,其作用机制可能与C-Jun基因的表达有关。
Objective： To investigate the effect of human cytomegalovirus（HCMV） infection on the development of colorectal adenocarcinoma and its possible mechanisms.Methods： RT-PCR was used to detect the expression of immediate-early gene 2（IE2） mRNA to reflect HCMV infection while the expression of C-Jun in 84 colorectal adenocarcinoma tissues and adjacent tissues was evaluated with immunohistochemical straining.Results： The positive rate of HCMV IE2 in colorectal adenocarcinoma tissues was higher than that in the adjacent tissues（P0.01）;The positive rate of C-Jun in colorectal adenocarcinoma tissues was also higher than that in the adjacent tissues（P0.01）;The expression of C-Jun gene in colorectal adenocarcinoma tissues corelated with HCMV infection（P0.05）.Conclusion： HCMV infection may play an important role in the development and progression of colorectal adenocarcinoma,and its mechanisms may be related with the expression of C-Jun.
出处
《现代生物医学进展》
CAS
2013年第15期2951-2954,共4页
Progress in Modern Biomedicine
基金
山东省自然科学基金项目(Y2007C030)
