摘要
目的分离和鉴定尿路上皮癌患者的肿瘤组织和抗凝血标本中人巨细胞病毒(HCMV),结合临床资料分析比较HCMV活动性感染与尿路上皮癌发生发展及其复发的相关性。方法采集33例经病理检测确诊为尿路上皮癌患者的组织和血标本,进行如下处理:分别采用细胞培养病毒分离法进行病毒分离;PCR法检测患者的抗凝血及组织DNA标本中HCMV UL55和UL83基因;IFA法检测抗凝血标本中HCMV pp65抗原;ELISA法检测HCMV IgG及IgM抗体;记录分析结果,确定尿路上皮癌患者HCMV活动性感染率。结果在33份抗凝血标本中,分离到1株临床分离株,该标本经盲传3代出现典型HCMV特征性细胞病变效应(CPE),经鉴定为HCMV。HCMV UL55和UL83基因、HCMV pp65抗原血症及HCMV IgM抗体检测均为阳性的有6份,因此可以判定在33例尿路上皮癌患者中,HCMV活动性感染者有6例,活动性感染率为18.2%(6/33)。结合患者的临床资料进一步分析发现,尿路上皮癌患者复发及病理分级均与HC-MV活动性感染相关,且HCMV活动性感染者其病理分级程度升高。结论结合临床资料分析发现,尿路上皮癌复发及病理分级程度升高均与HCMV活动性感染有关。
Objective To isolate and identify the HCMV among the cancerous tissue and anticoagulant blood from the patients with urothelial carcinoma,and to analyze the correlation of HCMV activated infection and urothelial carcinoma progression,especially recurrence.Methods The tissue and blood samples were collected in 33 patients who were identified as urothelial carcinoma with pathologic diagnosis.We performed the cell culture to isolate the virus,the PCR to detect the HCMV UL55 and UL83 gene,the IFA to identify the HCMV pp65 antigen among blood samples,and the ELISA to detect the HCMV IgG and IgM,and analyzed the data.Moreover,to identify the HCMV active infection rate in patients with urothelial carcinoma.Results There was one clinical isolate line among 33 anticoagulant specimens,and the specimen was characteristic of HCMV CPE via three generations,which was identified as HCMV.There were 6 specimens positive with HCMV UL55,UL83 gene,HCMV pp65 antigenemia and HCMV IgM,so we determined HCMV active infection cases were 6 and active infection rate was 18.2%（6/33）.We further demonstrated recurrence and pathological grade were both related to HCMV active infection and the pathological grading was elevated in HCMV active infector.Conclusion The recurrence and pathologic further grading of urothelial carcinoma is closely linked with HCMV active infection.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第1期60-65,共6页
Acta Universitatis Medicinalis Anhui
