摘要
选择野生型P5 3缺失型的Jurkat细胞系 ,首先将wt p5 3 pLXSN稳定转染入Jurkat细胞 (Jurkat wt p5 3) ,再分别或联合转染IE1、IE2基因 ,并利用流式细胞仪分析。结果显示 ,转染了IE2基因的Jurkat wt p5 3细胞在瞬时转染 2 4h后出现光镜下可见的细胞凋亡改变 ,此时取样进行流式细胞仪测定细胞周期分布状况 ,可见转染了IE2基因的Jurkat wt p5 3细胞周期分布较其他各组发生了明显的变化 ,G1期和S期细胞分布减少 ,而G2 期细胞增多 ,细胞阻滞于G2 /M期 ,并且出现了细胞凋亡亚二倍体峰 ,引起细胞凋亡。研究表明 ,在Jurkat细胞中HCMV的IE2基因可介导野生型p5 3基因的促凋亡作用。
To investigate the function of immediately early gene 1 and 2(IE1 and IE2) of human cytomegalovirus on p53 gene in Jurkat cell line that lacks p53 protein expression, gene transfer approach and flow cytometry were used. A p53 expressed plasmid named wt p53 pLXSN was first introduced stably into Jurkat cells, and then IE1 and IE2 expressed plasmids were transfected transiently respectively. After 24 hours, apoptosis in the group of Jurkat cells transfected with p53 and IE2 was found.The analysis of flow cytometry showed that the cells of G 1 and S phases were reduced, most of cells were arrested in G 2/M phase and sub G peak emerged in this group.The results suggest that the IE2 gene of human cytomegalovirus may mediate the pro apoptotic function of p53 gene in Jurkat cells.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第6期522-524,共3页
Medical Journal of Chinese People's Liberation Army
基金
全军医学科研"九五"计划重大项目资助课题 (编号 98D0 4 6)
陕西省自然科学基金 (编号 98M38)资助课题
