摘要
从土壤中分离到９ 株产生β甘露聚糖酶的芽孢杆菌（ Ｂａｃｉｌｌｕｓ ｓｐ ．） 。Ｂａｃｉｌｌｕｓｓｐ ． Ｍ５０２５０ｍ Ｌ三角瓶摇瓶培养试验，以４ ％ 的魔芋粉为碳源，１－０ ％ （ ＮＨ４）２ＳＯ４ 为氮源，０－３５ ％Ｎａ２ＣＯ３ ，３０ ～３４ ℃培养６０ｈ 产酶达到高峰。酶活力为１８０ ～２００ｕ／ｍ Ｌ。１００Ｌ 罐发酵，在３０ ～３２ ℃，１∶０ ．７５ｖｖｍ 通气量，２００ｒ／ｍｉｎ 条件下，发酵液酶活力高达３３０ｕ／ｍ Ｌ。酶的最适反应温度和ｐＨ 分别为５０ ℃和６－０ ，低于５０ ℃，ｐＨ５ ．０ ～７ ．０ 酶稳定。Ｆｅ３＋ 、Ａｌ３ ＋ 、ＥＤＴＡ、Ｈｇ２＋ 对酶有抑制作用，而Ｂａ２ ＋ 、Ｍｎ２＋ 对酶有激活作用。发酵粗酶液对苎麻精干麻精练，显示对精干麻的半纤维素残胶具有降解作用。
Bacillus\% sp. M\-\{50\} produced β mannanase efficiently in the medium composed of 4% konjac powder, 1.0% (NH\-4)\-2SO\-4 and 0.35% Na\-2CO\-3.Fermentation of β mannanase by \%Bacillus\% sp. M\-\{50\} has been carried out in 100L tank. The temperature, ventitation quantity and agitation speed were 30-32℃, 1:0.75 vvm and 220r/min respectively. the fermentation cycle was 28 h and the highest β mannanase activity was 330 u/mL. The optimal temperature and pH for β mannanase reaction were 50℃ and 6.0 respectively. It was stable at pH 4.0-7.0 and below 50℃. The activity of the enzyme was strongly inhibited by Fe 3+ ,Al 3+ ,EDTA, Hg 2+ and slightly stimulated by Ba 2+ , Mn 2+ .Treatmet of \%ramie-fibers\% with crude enzyme preparations from \%Bacillus\% sp. M\-\{50\} showed a good degradation for hemicellulose of ramie\|fibers residues.
出处
《微生物学报》
CAS
CSCD
北大核心
2000年第1期62-68,共7页
Acta Microbiologica Sinica
基金
四川省"九- 五"攻关项目
中国科学院地奥科学基金
