摘要
由我国内蒙乌杜淖碱湖中分离到一株能产β-甘露聚糖酶的嗜碱芽孢杆菌(AlkaliphilicBacillus sp.),产酶的最适培养基为(%):槐豆胶2.0,谷氨酸钠1.5,K_2HPO_4 0.1,MgSO_4·7H_2O 0.02,NaCl 8.0及 Na_2CO_31.0。产酶的最适温度和 pH 分别为32℃和10.0,酶的最适反应温度和 pH 分别为70℃和9.5—10.0,该酶在 pH9.0—10.0范围内稳定。酶对魔芋粉和槐豆胶的水解产物主要为寡糖。
An alkalophilic Bacillus sp.producing β-
mannanase was isolated from Wudunor soda
lake of China.The optimal medium for the
enzyme production was composed of 2% locust
bean gum,1.5% sodium glutamate,0.1%
K_2HPO_4,0.02% MgSO_4·7H_2O,8%NaCl
and 1.0% Na_2CO_3,The optimal cultiva-
tion temperature and initial pH for the
enzyme production were 32℃ and 10.0
respectively.The optimal pH and temp-
erature for the enzyme reaction were
9.5—10.0 and 70℃,respectively.The
hydrolysis products of konjac powder
and locust bean gum with the enzyme
were a series of oligosaccharides.
出处
《微生物学报》
CAS
CSCD
北大核心
1991年第6期443-448,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金
