摘要
目的通过对乙型肝炎肝硬化、慢性乙型肝炎和健康人血清中Treg和Th17细胞及其相关因子IL-17、IL-21和IL-10的检测,结合血清ALT、TBA水平、HBV-DNA载量及HBeAg定量检测,观察各细胞因子之间的相关性,进一步探讨3种细胞因子在HBV感染患者体内的平衡关系。方法实验共分为4组:乙型肝炎肝硬化组(LC,38例),轻中型慢性乙型肝炎组(CHBLM,35例),重型慢性乙型肝炎组(CSHB,36例)和健康对照组(HC,40例)。采用流式细胞术(FCM)检测各组外周血Treg和Th17表达率。采用酶联免疫法(ELISA)检测血清中IL-17、IL-21和IL-10含量;RT-PCR方法检测患者HBV-DNA载量;电化学发光法检测HBeAg含量;全自动生化分析仪检测ALT和TBA。对得到的数据进行相关性检测。结果 1)3组HBV感染患者血清Treg和Th17细胞及IL-21和IL-10水平均显著高于健康对照组(P<0.05);2)IL-10与HBV-DNA载量呈正相关(r=0.727,P<0.05),IL-21、IL-17与ALT和TBA相关(P<0.01);3)HBV感染患者Treg/Th17、IL-10/IL-17、IL-10/IL-21值与健康对照组相比差异有统计学意义(P<0.05)。结论 1)Treg、Th17细胞及IL-17、IL-21和IL-10在HBV感染者不同病程过表达;2)IL-10与HBV载量呈正相关,IL-17、IL-21与HBV载量无相关性;3)IL-17、IL-21与反映肝细胞损害的生化指标ALT与TBA呈正相关;4)IL-17与IL-21在CSHB患者和LC患者呈正相关;5)不同病程HBV感染患者存在Treg/Th17、IL-10/IL-17、IL-10/IL-21失衡。
Objective To investigate the significance of Thl7/Treg and explore the role of the balance of three associat- ed cytokines, IL-17, IL-21, and IL-10, in the development of hepatitis B, IL-17, IL-21, and IL-10 were detected in sera from patients with liver cirrhosis, patients with chronic hepatitis B, and healthy individuals, and the association between these cytokines and ALT, TBA, HBV DNA capacity, and hepatitis B e antigen was examined. Methods Sera came from four groups of subjects： patients with liver cirrhosis （LC, n=38）, patients with chronic hepatitis B of a low or moderate grade （CHBLM, n：35）, patients with severe chronic hepatitis B （CSHB, n= 36）, and healthy individuals （HC, n= 40）. The percentage of Treg and Thl7 in peripheral blood was detected with flow cytornetry analysis. Levels of IL-17, IL-21, and IL-10 were detected with ELISA, HBV DNA was detected with RT-PCR, levels of ALT and TBA were detected with an automated biochemistry analyzer; and HBeAg was detected by electro-chemiluminescence. The re lationship between these factors was then analyzed. Results （1） Patients with chronic hepatitis B had significantly more Treg and Thl7 and higher levels of their associated cytokines IL-21 and IL-10 in serum than did healthy individuals （P〈 0.05）. （2） IL-10 was correlated with the viral load of HBV DNA （r=0. 727, P〈0.05）. （3） IL-21 and IL-17 were cor related with ALT and TBA. （3） Patients with chronic hepatitis B had significantly different levels of Th17/Treg and IL- 10/IL-17 and IL-10/IL-2 than did healthy individuals. Conclusions （1） Treg and Th17 and their associated cytokines IL-17, IL-21, and IL-10 were overexpressed in patients with chronic hepatitis B. （2） IL-10 was correlated with the viral load of HBV DNA while IL-17 and IL-21 were not. （3） IL-17 and IL 21 were correlated with ALT and TBA while IL-10 was not. （4） IL-17 was correlated with IL-21 in patients with CSHB and patients with LC. （5） The equilibrium of Th17/ Treg, IL-10/IL-17, and IL-10/IL-21 was disrupted in patients with chronic hepatitis B.
出处
《中国病原生物学杂志》
CSCD
北大核心
2013年第4期310-314,共5页
Journal of Pathogen Biology
基金
新疆自治区重点学科-病原生物学建设项目(No.xj100103)
