摘要
目的:研究自然状态下献血者对乙型病毒性肝炎不同抗原成份的体液、细胞免疫应答水平,及两者的相关性。方法:ELISA检测献血者血浆中HBsAb、HBcAb、preS1+S2-Ab的阳性率,ELISOPT检测外周血单个核细胞中抗原特异性IFN-γ分泌细胞水平。结果:体液免疫应答检测结果表明preS1+S2-Ab的检出率最高,占85.7%(48/56),核心杭体的检出率最低30.4%(17/56);特异性细胞免疫检测结果提示献血者总体preS1+S2-Ag、HBcAg的特异性细胞免疫应答水平有着良好的一致性,无统计学差异(P>0.05),但显著优于HBsAg;preS1+S2-Ag和HBsAg特异性细胞免疫和体液免疫应答有着较好的一致性,但HBcAg特异性细胞免疫和体液免疫无明确关联(P>0.05)。结论:自然状态下献血者对乙型病毒性肝炎不同抗原成份均有较好的体液、细胞免疫应答,preS1+S2的体液免疫应答水平优于表面及核心抗原提示人HBV疫苗的侯选对象。
AIM： To study the magnitude and correlation of humoral and cellular immune responses to hepatitis surface antigen（HBsAg）, preS1 ＋ S2 antigen, and core antigen （HBcAg） in the blood donors. METHODS： Enzyme linked immunosorbent assay （ELISA） was employed to scan the humoral immune responses to HBsAg, preS1 ＋ S2 antigen, and HBcAg. Enzyme linked immunospot assay （ELISPOT） was used to check the antigen specific IFN-γ, secreting cells stimulated by HBsAg, preS1 ＋ S2 antigen or HBcAg in peripheral blood mononuclear cell from blood donors. RESULTS： The prevalence of humoral immune response to preS1 ＋ S2 antigen was as high as 85.7% （48/ 56）, compared with the lower response to HBcAg （30.4%, 17/56）. In the cellular immune response, there was no difference between preS1 ＋ S2 antigen and HBcAg, both of which were stronger than the response stimulated by HBsAg. PreS1 ＋ S2 antigen or HBsAg specific humoral immune and cellular immune responses were highly correlated, which could not be observed in the HBcAg specific humoral immune and cellular immune responses （ P 〉 0.05）. CONCLUSION： Either humoral or cellular immune responses to HBsAg, preS1 ＋ S2 antigen and HBcAg could be detected in the blood donors. PreS1 ＋S2 antigen immune responses, however, were most powerful, which may suggest that PreS1 ＋ S2 antigen may be a candidate for the further HBV vaccine.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2012年第4期404-406,共3页
Chinese Journal of Cellular and Molecular Immunology
