摘要
目的 探讨脾虚证与胃窦及十二指肠组织的 5 - HT及其受体的关系 .方法 成年 SD大鼠 30只随机分为 3组 .A组 :脾虚组 ( n=10 ) ;B组 :对照组 ( n=10 ) ;C组 :四君子汤治疗组 ( n=10 ) .采用免疫组化和医学图像分析系统相结合的方法 ,测定各组大鼠胃窦及十二指肠 5 - HT细胞的数目、面积、平均灰度、细胞内 5 - HT含量及 5 - HT受体阳性细胞内 5 - HT受体的含量 .结果 1A组大鼠胃窦 5 - HT细胞的数目 ( 10 6±8) .HP- 1 ,面积 ( 35 .1± 4.0 )μm2 ,5 - HT含量 ( 0 .337± 0 .0 5 0 )AU,5 - HT受体含量 ( 0 .345± 0 .0 75 ) AU均高于 B组 ( P<0 .0 5或 P<0 .0 1) ,平均灰度 ( 12 0± 8)较 B组降低 ( P<0 .0 5或P <0 .0 1) ;A组大鼠十二指肠 5 - HT细胞的数目 ( 10 9± 5 ) .HP- 1 ,面积 ( 30 .2± 2 .2 )μm2 ,5 - HT含量 ( 0 .341± 0 .0 31)AU ,5 - HT受体含量 ( 0 .319± 0 .0 31) AU均高于 B组 ( P<0 .0 1) ,平均灰度 ( 12 3± 4)较 B组降低 ( P<0 .0 1) . 2 C组的上述指标测定结果与 B组无显著差异 .结论 胃窦及十二指肠组织 5 - HT及其受体含量的增高可导致消化系统功能紊乱 。
AIM To study the relationship of spleenasthenia syndrome with 5hydroxytryptamine (5HT) and 5HT receptor in gastric antral and duodenal tissues. METHODS Thirty SD adult rats were divided into three groups at random. Group A: spleenasthenia group (n=10); group B: control group (n=10); group C: treated by sijunzi decoction group (n=10). The immunohistochemistry and MIPS were used to measure 5HT cell number, square, mean grey level and the contents of 5HT and 5HT receptor in their positive cells in every rat's gastric antrum and duodenum. RESULTS ①In group A, rat gastric antrum 5HT cell number (106±8)·HP -1 , square (35.1±4.0) μm2, the contents of 5HT (0.337±0.050) AU, the contents of 5HT receptor (0.345±0.075) AU were all higher than those in group B (P< 0.05 or P<0.01), mean grey level (120±8) was lower than that in group B (P<0.05 or P<0.01); In group A, rat duodenum 5HT cell number (109±5)·HP -1 , square ( 30.2 ±2.2) μm2, the contents of 5HT (0.341±0.031) AU, the contents of 5HT receptor (0.319±0.031) AU were all higher than those in group B (P< 0.01 ), mean grey level (123±4) was lower than that in group B (P<0.01).②The measured parameters as above in group C didn't change significantly compared with those in group B (P>0.05). CONCLUSION The increase of 5HT and 5HT receptor's contents in gastric antrum and duodenum would lead to disturbance of the function of digestive system, it may be a kind of important pathogenesis or pathology of spleenasthenia syndrome.
出处
《第四军医大学学报》
2000年第5期S087-S089,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目!(39270864)
关键词
脾虚证
羟色胺
受体
幽门窦
十二指肠
spleen-asthenia syndrome
5-hydroxytryptamine
5-hydroxytryptamine receptor
pyloric antrum
duodenum
