摘要
目的建立测定三七胶囊中5种皂苷含量的超快速液相色谱法。方法以Shimadzu C18(75 mm×2.0mm,2.2μm)为色谱柱,柱温25℃,乙腈-水梯度洗脱,流速0.3 mL.min-1,检测波长203 nm。结果三七皂苷R1在9.38～351.62 ng(R2=1),人参皂苷Rg1在52.11～1 954.12 ng(R2=0.999 9),人参皂苷Re在6.85～152.17 ng(R2=0.9996),人参皂苷Rb1在44.99～1 687.25 ng(R2=1),人参皂苷Rd在9.96～221.27 ng(R2=1)范围线性关系良好;加样回收率分别为96.6%(RSD=0.9%),97.1%(RSD=1.4%),101.0%(RSD=1.4%),97.9%(RSD=2.4%),98.2%(RSD=1.8%)。结论该方法简单,快速,适用于测定三七胶囊中五种皂苷含量。
Objective To establish an ultra fast liquid chromatography （UFLC） method for determining five saponins in sanqi capsule. Methods Chromatography was performed on an shimadzu C18 column （75 mm×2.0 mm, 2.2 μm） at 25 ℃. The gradient elution was acetonitrile-water at a flow rate of 0.3 mL × min^-1. The detection wavelength was at 203 rim. Results The linear ranges for notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd were 9.38-351.62 ng （R^2=1）, 52.11-1 954. 12 ng （R^2 =0.999 9）, 6.85-152.17 ng （R^2 =0. 999 6）, 44.99-1 687.25 ng （R^2 = l ）, 9.96-221.27 ng （R^2 = 1 ）, respectively. The average recoveries were 96.6% for notoginsenoside RI（ RSD=0.9% ）, 97.1% for ginsenoside Rg1 （ RSD = 1.4% ）, 101.0% for ginsenoside Re （ RSD = 1.4% ）, 97.9% for ginsenoside Rb1 （ RSD = 2.4%）, 98.2% for ginsenoside Rd （RSD=1.8%）, respectively （n=6）. Conclusion The method is simple, rapid and suitable for determination of five saponins in sanqi capsule.
出处
《医药导报》
CAS
北大核心
2013年第1期81-84,共4页
Herald of Medicine
