摘要
讨论了醋酸铵对高效液相色谱法（ＨＰＬＣ）分离人参单体皂甙的色谱改性情况，经试验选择采用ＭＰＧ－ＯＤＳ色谱柱，ＵＶ２０４ｎｍ处检测，以乙腈－水－醋酸铵为流动相，在１５分钟内较好地分离了单体皂甙Ｒ＿（ｇ２）、Ｒ＿（ｂ１）、Ｒ＿ｃ、Ｒ＿ｄ、Ｒ＿（ｇ１）、Ｒ＿ｅ等。方法操作简单、快速，可用于人参质量评定的常规分析。
he chromatographic properties of ammonium acetate in HPLC mobile phase for ginsenosides assay havebeen studied, and six ginsenosides(R_(g2),T_(b1),R_c,R_d,R_(g1),R_e)were separated by the method using a column ofoctadecylsilyl chemically modified porous glass(MPG-ODS)within 15 minutes and eluted with acetonitrile-waterammonium acetate with UV detector at 204nm.The ammonium acetate in this eluant improved the separatingdegree of ginsenosides in crude drugs.This method is simple,rapid and accurate,It can be used for assaying ginseng raw materials and ginsengpreparatlons et al.
出处
《色谱》
CAS
CSCD
北大核心
1994年第1期40-42,共3页
Chinese Journal of Chromatography
基金
国家自然科学基金与高校博士学科点专项科研基金资助课题
关键词
高效液相色谱
醋酸铵
人参单体皂甙
RP-HPLC
mobile phase
modifier
ammonium acetate
ginsenosides R_(g2),R_(b1),R_c,R_d,R_(g1),R_e
