摘要
目的测定三七药材中三七皂苷的含量。方法采用高效液相色谱法C18色谱柱,乙腈∶水(15∶8512min30∶7045min15∶85v/v)梯度洗脱,检测波长203nm。结果三七R1在72.8～109μg·ml1的范围内(r=0.9991),人参Rg1在68.8～860μg·ml1的范围内(r=0.9992),Rb1在62.8～785μg·ml1的范围内(r=0.9998),线性关系良好。平均回收率大于98%,方法的变异系数小于3.0%。结论方法简便,快速,准确,重现性好,可用于三七药材中三七皂苷的含量测定。
Objective： A rapid high--performance liquid chromatographic method to determine the contents ginsenoside Rg1 and Rb1 and notoginsenoside R1 of Panax notoginseng was described. Methods： Agilent Hypersil C18 column was used, with mixture liquid of acetonitrile and water （15 ： 85-12 min→30 ： 70-45 min→15 ： 8～5 v/v） as mobile phase in a gradient mode. The wavelength of measurement was set at 203 nm. Results：The linear range of this method was 72.8～109μg. ml^-1 for （R1） （r=0. 999 1）, 68.8～860μg· ml^-1 for （Rg1）（r=0. 999 2）, 62.8～785μg· ml^-1 for （Rb1）（r=0. 999 8） respectively. The coefficient of variation was smaller than 3.0% （n= 5）. Conclusion：The method had the advantages of simplicity, rapidity, accuracy and precision, and can be used to identify and evaluate the quality of Panax notoginseng.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2005年第8期710-711,共2页
Lishizhen Medicine and Materia Medica Research
基金
广州市应用基础研究项目(2001J01301)
