摘要
目的:建立七丹胶囊中4个成分的高效液相色谱测定方法。方法:采用汉邦C18柱(150mm×4.6mm,5μm);以乙腈-水为流动相,进行梯度洗脱,流速:1.0mL/min,检测波长为203nm进行三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1皂苷有效成分含量测定。采用汉邦C18柱(150mm×4.6mm,5μm);流动相(甲酸-水)-(甲醇-乙腈)=62∶38,其中甲酸-水(1∶59),甲醇-乙腈(30∶10),流速:1.0mL/min,检测波长:286nm,进行丹酚酸B含量测定。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1、丹酚酸B线性范围分别为0.4721～2.832μg(r=0.9995)、1.734～10.404μg(r=0.9999)、1.732～10.392μg(r=0.9999)、0.4～4.0μg(r=0.9999);平均加样回收率(n=5)分别为100.32%、99.965%、100.285%、101.4%;RSD分别为1.01%、2.53%、2.46%、1.88%。结论:本测定方法简便可行、重复性好,可用于七丹胶囊中4个成分的含量测定。
AIM： To establish the method for determing four effective components in Qidan Capsules（total saponin of Radix et Rhizoma Notoginseng,total phenolic acids of Radix et Rhizoma Salviae Miltiorrhizae） by RPHPLC. METHODS： The contents of ginsenoside Rg1、ginsenoside Rb1,notoginsenoside R1 were simultaneously determined by an HPLC system with Lichrospher C18（150 mm × 4. 6 mm,5 μm）. The mixture of acetonitrile and water was used as the mobile phase and the flow rate was 1. 0 mL/min. The detection wavelength was set at 203 nm. The content of salvianolic acid B was determined by an HPLC system with Lichrospher C18 （150 mm × 4. 6 mm,5 μm）. The mobile phase was （formic acid-water）-（methanol-acetonitrile） = 62 ∶ 38. Formic acid-water （1 ∶ 59）,Methanol-acetonitrile （30 ∶ 10）,and the flow rate was 1. 0 mL/min and the detection wavelength was 286 nm. RESULTS： The linear ranges of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 and salvianolic acid B were 0. 472 1 - 2. 832 μg（r = 0. 999 5）,1. 734 - 10. 404 μg （r = 0. 999 9）,1. 732 - 10. 392 μg （r = 0. 999 9）,0. 4 - 4. 0 μg （r = 0. 999 9） respectively. The average recoveries （n = 5） were 100. 32% ,99. 965% , 100. 285% and 101. 4% ,corresponding RSD were 1. 01% ,2. 53% ,2. 46% and 1. 88% respectively. CON-CLUSION： The results indicate that the HPLC method is simple,highly selective and reproducible; thus it can be used in the determination of the four components in Qidan Capsule.
出处
《中成药》
CAS
CSCD
北大核心
2010年第5期782-786,共5页
Chinese Traditional Patent Medicine
基金
云南省新药研发专项(2003XY11)
