摘要
目的:探讨中国沙棘粗多糖中α-葡萄糖苷酶抑制剂的体外酶动力学的特性。方法:利用酶-抑制剂筛选模型,选择沙棘粗多糖,通过改变其浓度、反应时间、pH条件、温度等进行体外酶学动力学研究。结果:沙棘粗多糖浓度在0.0098～0.625 g.L-1之间时,酶的抑制率随着浓度的增大而升高,IC50为0.031 g.L-1;浓度为0.625 g.L-1时抑制率达92.52%,且与酶结合迅速,酸碱范围广,热稳定性好。结论:沙棘粗多糖是一种高活性的竞争可逆性α-葡萄糖苷酶抑制剂,抑制常数Ki=5.26g.L-1。
Objective：To investigate the characteristics of in vitro enzyme kinetics of α - glucosidase inhibitors in crude polysaccharide of Chinese seabuckthorn （Hippophae rhamnoides L. ssp. sinensis Rousi）. Method：Highly ac- tive crude polysaccharide of Seabuckthorn was selected by using enzyme - inhibitor screening model, and the in vitro enzyme kinetics was investigated by changing its concentration, reaction time, pH conditions, temperature and oth- ers. Results ： When Seabuckthorn polysaccharide concentration is between 0. 0098 g·L^-1 to 0. 625 g·L^-1, the en- zyme inhibitory rate positively correlates with the concentration, IC50 for 0.031 g·L^-1 ; when the concentration rea- ches 0. 625 g·L^-1, and inhibitory rate is up to 92.52% ;Seabuckthorn polysaccharide rapid integrates with the en- zyme, and has wide pH ranged and good thermal stability. Conclusion：Crude polysaccharide of Seabuckthorn is a highly active and competitive reversible inhibitor ofα - glucosidase, with inhibition constant Ki = 5.26 g·L^-1.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第12期2154-2157,共4页
Chinese Journal of Pharmaceutical Analysis
基金
青海省自然科学基金项目(2012-Z-909)
关键词
Α-葡萄糖苷酶
抑制剂
沙棘粗多糖
酶-抑制剂筛选模型
酶动力学
α - glucosidase
inhibitor
crude polysaccharide of Seabuckthorn
enzyme - inhibitor screening model
enzyme kinetics
