摘要
采用响应曲面分析法对超声波提取灵芝多糖的工艺条件进行优化,优化后的工艺为:超声功率320W,提取温度70℃,提取时间34 min,其多糖得率为2.78%,氧自由基吸收能力(ORAC值)为1956μmolTrolox/g,与理论预测值无显著性差异。因此,采用响应面分析法优化灵芝多糖超声提取工艺,具有较好的可行性。在最佳条件下,制备的灵芝多糖分子量组成为18150 Da(20.07%)和6484 Da(79.93%),大大低于传统水提灵芝多糖的分子量。
The response surface methodology was used to optimize the ullrasonic extraction of Ganoderma lucidum polysaccharides. Design expert analysis indicated that the optimal processing conditions were： ultmsounic power 320 W, temperature 70 ℃ and extraction time 34 rnin, respectively. The extraction yield and the ORAC value of polysaccharide were 2.78% and 1956 lamol Trolox/g, respectively, which had no significant differences with the predicted values, indicating that the response surface methodology was a reasonable method to optimize the extraction conditions of Ganoderma lucidum polysaccharides. The molecular weights dislribution of the polysacchaddes under the optimal conditions were 18150 Da （20.07%） and 6484 Da （79.93%）, much less than those of the polysaccharides prepared by traditional water extraction.
出处
《现代食品科技》
EI
CAS
北大核心
2012年第12期1704-1708,1773,共6页
Modern Food Science and Technology
基金
广东省部产学研项目(2010A090200041)
广东省科技计划项目(2011B030500004)
国家自然科学基金(31101222)
企业横向项目"灵芝多糖构效关系及工艺研究"资助
关键词
灵芝
多糖
得率
抗氧化活性
分子量
Ganoderma lucidum
polysaccharides
extraction yield
antioxidant activity
molecular weight distribution.
