摘要
利用HPLC法研究了灵芝真菌发酵过程中胞内外麦角固醇的含量,经紫外全波长扫描,确定测定波长为282nm,研究表明:此方法测定麦角固醇含量最大标准偏差为0.92%,得到线性回归方程为y=28685x-382.61(R=0.9998),该回归方程在20～500μg的范围内。精密度试验,灵芝真菌发酵胞内外麦角固醇含量RSD分别为0.40%和1.96%,说明此试验设计条件精密度良好,经加标后,麦角固醇的回收率在96.82～102.50%之间,表明此方法用于测定灵芝真菌发酵过程中麦角固醇含量准确、可行,经测定灵芝真菌发酵至120h,麦角固醇含量增至最高,胞内含量达到0.64 mg/g,胞外含量达到0.07 mg/mL。
The ergosterol content in fermentation ofGanoderma lucidum was determined by HPLC at 282 nm. The results showed that the maximum standard deviation was 0.92% and the linear regression equation y= 28685x-382.61 （R=0.9998） in the range of 20～500 pg. The precision test showed that Ganoderma lucidum extraceUular and intracellular ergosterol content of RSD were 0.40% and 1.96%, respectively. The spike recoveries for ergosterol were between 96.82 and 102.50%. The method was feasibility and accurate for determination of ergosterol content in fermentation of Ganoderma lucidum. The ergosterol content of Ganoderma lucidum extracellular and intracellular were 0.07 mg/mL and 0.64mg/g, respectively, after fermentation for 120 h.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第9期2267-2270,共4页
Modern Food Science and Technology
基金
衡水学院教改课题项目(jg2012061)
