摘要
【目的】利用二次回归正交旋转组合设计优化复合酶法提取甘蔗梢多酚工艺条件,为甘蔗梢的综合利用提供技术参考。【方法】以粤糖159号蔗梢为试验原料,在单因素试验基础上,以甘蔗梢多酚提取量为考察指标,选择复合酶用量、p H、提取温度、提取时间为试验因素,采用4因子二次回归旋转组合设计(1/2实施)对甘蔗梢多酚提取工艺进行优化,确定复合酶法最佳工艺条件。【结果】建立甘蔗梢多酚提取量(Y)对复合酶用量(X1)、p H(X2)、提取温度(X3)及提取时间(X4)4个试验因素的二次正交回归模型:Y=4.51155+0.16908X1+0.20925X3-0.14736X32,该模型拟合性较好,其中复合酶用量和提取温度对甘蔗梢多酚提取量有极显著影响(P<0.01)。复合酶法提取甘蔗梢多酚的最佳工艺条件为:在复合酶(果胶酶∶纤维素酶=2∶3)用量1.6%、p H 5.0、提取温度56℃的条件下提取30 min,甘蔗梢多酚提取量的模型预测值为4.86 mg/g,重复3次试验的验证值为4.71 mg/g;通过检验分析,显著性水平为0.01时,验证值与预测值一致。【结论】采用二次回归正交旋转组合设计优化得到的复合酶法提取工艺条件准确可靠,有效提高了甘蔗梢多酚提取效率,可在实际生产中推广应用。
【Objective】The compound enzymatic extraction process of polyphenols from sugarcane top were optimized by quadratic regression orthogonal rotation combination design, in order to provide references for comprehensive utilization of sugarcane top. 【Method】Using sugarcane top of Yuetang 159 as raw materials and extracted yield as index, based on single factor experiments, four factors（compound enzymatic dosage, p H, extraction temperature, extraction time） were selected as four experimental factors, and the four-factor quadratic regression orthogonal rotation combination design（1/2implemented） was used to optimize extraction process of polyphenols from sugarcane top, so as to ascertain optimal compound enzymatic extraction process conditions. 【Result】The quadratic orthogonal regression model of extracted polyphenols yield（Y） for compound enzymatic concentration（X1）, p H（X2）, extraction temperature（X3） and extraction time（X4） was Y =4.51155 ＋0.16908X1＋0.20925X3-0.14736X32, with good fitting degree. Among four factors, the compound enzymatic concentration and extraction temperature had extremely significant effects on yield of polyphenols extracted from sugarcane top（P0.01）. Furthermore, the optimal compound enzymatic extraction process conditions were as follows： compound enzymatic dosage of 1.6%（pectinase∶cellulose=2∶3）, p H of 5.0, extraction temperature of 56 ℃, and extraction time of 30 min. Under optimal conditions, the model predicted value of polyphenols yield was 4.86 mg/g, the verified value of extracted polyphenols yield based on experiments repeated 3 times was 4.71 mg/g. The results of significance test showed that, the verified value had no significant difference from predicted value at 0.01 level. 【Conclusion 】It is accurate and reliable that the quadratic regression orthogonal rotation combination design is used to optimize compound enzymatic extraction process conditions, and the extraction efficiency of polyphenols from sugarcane top can be improved effectively,so it can be applied in the actual production.
出处
《南方农业学报》
CAS
CSCD
北大核心
2016年第3期459-465,共7页
Journal of Southern Agriculture
基金
国家自然科学基金项目(20110831)
广西高校科学技术研究重点项目(ZD2014004)
关键词
多酚
甘蔗梢
复合酶法
正交旋转组合设计
工艺优化
polyphenols
sugarcane top
compound enzymatic method
orthogonal rotation combination design
process optimization
