摘要
本文建立了高效液相色谱法定量测定食品中的单糖、双糖,确定的色谱条件如下:WatersNH2氨基色谱柱(250mm×4.6mm),乙腈:水85:15(V/V)为流动相,流速1.0ml/min,示差折光检测器,40℃柱温,进样量10μl,外标法定量。该方法的精密度RSD<5%,回收率大于97%。在此色谱条件下20min内完成了单糖、双糖的分离,结果比较理想。研究表明此新建方法准确而快速,并能适用于多数食品中单糖、双糖的测定。
HPLC method was established to determine the monosaccharide and disaccharide in foods. The chromatographic conditions are as belows： Waters amino chromatographic column with refractive index detector, ehitioninfluent methane：water （V/V） 85：15, flow rate 1.0ml/min and injection volume 10 μl. The repeatability relative standard deviation of this method is below 5% and the recovery is over 97%. A satisfsacfory result is obtained under these optimum conditions, and the whole separation can be finished with in 20min. The results showed that this new HPLC analysis method is both correct and fast enough to be used in most kinds of foods contained monosaccharide and disaccharide.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第3期293-296,共4页
Food Science
关键词
高效液相色谱
单糖
双糖
食品
HPLC
monosaccharide and disaccharide
foods
