摘要
目的 建立人成骨肉瘤细胞系。方法 采用原代组织块培养法对成骨肉瘤手术标本进行培养 ,对存活细胞进行形态学观察、组织化学染色、乳酸脱氢酶 (LDH)同工酶谱、细胞周期检查、核型分析、异种移植。结果 建立细胞系SOSP 96 0 7,其形态学表现、组织化学染色、LDH同工酶谱等均符合成骨肉瘤的特征。经 1年体外培养 ,已连续传代 12 0次 ,细胞倍增时间 2 9.8h ,细胞周期测定G1期为 48.2 %、G2 期为 2 0 .9%、S期为 30 .9%。染色体具有亚三倍体核型 ,众数为 6 4～ 6 6条。异种移植成瘤率 10 0 % ,无支原体污染。结论 人成骨肉瘤细胞系SOSP 96 0 7可用于对成骨肉瘤的研究。
Objective\ To establish a human osteosarcoma cell line. Methods\ The specimens which were identified as osteosarcoma were cultured primarily in vitro. The surviving cells underwent morphological observation, histochemical staining, isoenzyme pedigree (LDH) determination, cell cycling analysis, karyotype analysis and heterotransplantation. Results\ The newly established cell line SOSP 9607 was maintained in continuous culture for over 120 transfer generations in 1 year. Morphological observation, histochemical staining and LDH isoenzyme pedigree revealed that SOSP 9607 had the common features of osteosarcoma. The cell cycle analysis showed:G1 48.2%, G2 20.9%, S 30.9%. The doubling time of cells was about 29.8 h. The chromosome analysis showed a hypotriplaid feature with a model number of around 64～66. The cells remained free off mycoplasma and the tumor formation ratio of heterotransplantion was 100%. Conclusion\ The SOSP 9607 appeared to be a cell line derived from human osteosarcoma cells and could be used as a model for further study on osteosarcoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第2期120-121,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金!( 3 93 3 0 2 0 0 )
