摘要
目的 建立人脊索瘤细胞系 ,为脊索瘤研究提供实验模型。方法 取经病理证实的新鲜脊索瘤手术标本 ,进行体外原代组织块培养。对存活细胞进行形态学观察、组织化学染色、细胞周期检查、染色体分析、电镜观察、异种移植和体外侵袭实验等。结果 建成细胞系CM 319,经近两年的体外培养 ,已连续传代百余次。其形态学表现、组织化学染色、电镜观察和异种移植等均符合脊索瘤细胞特征。细胞倍增时间为 33h。细胞周期测定显示 :G1 期为 5 5 .6 % ,G2 期为 2 1.9% ,S期为2 2 .5 % ,G2 G1 为 1.90。染色体具有亚三倍体核型。异种移植成瘤率 10 0 % ,具有侵袭性。结论 CM 319是一株人脊索瘤细胞系 ,可用于对脊索瘤的研究。
Objective To present an established human chordoma cell line for chordoma research. Methods The specimens pathologically identified as chordoma were cultured, using primary tissue culture in vitro. The surviving cells were analyzed by morphology, histochemical stain, cell cycling analysis, karyotype analysis, electron microscopic observation, heterotransplantation and study of invasive capacity in vitro. Results The newly established cell line CM 319 has been maintained in continual cultures for over 100 generations in two years. Its morphological observation, histochemical staining properties, electron microscopic observation and heterotransplantation showed the common characteristics of chordoma. The doubling time of cells was about 33 hours. Cell cycle analysis showed: G 1 55.6%, G 2 21.9% and S 22.5%, G 2/G 1=1.90. Chromosome analysis showed a hypotriploid feature and the success rate of heterotransplantation was 100%. It is capable of invasion in vitro. Conclusion CM 319, as a cell line derived from human chordoma cells, may serve for further studies of chordoma. [
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2003年第3期234-236,共3页
Chinese Journal of Oncology
