摘要
目的研究血小板源性生长因子-α受体沉默对人晶状体上皮细胞增殖及凋亡的影响，为治疗后发性白内障提供实验依据。方法体外培养人晶状体上皮细胞SRA01／04，用脂质体将合成的血小板源性生长因子—α受体反义寡核苷酸（PDGFR—α ASODN）处理SRA01／04，MTT法检测细胞的增殖；流式细胞仪检测细胞周期及细胞凋亡；Hoechst33258荧光染料染色法分析细胞凋亡；RT—PCR检测PDGF-α受体的表达。结果PDGFR—αASODN作用于SRA01／04细胞24h-72h，SRA01／04细胞增殖受抑制，且72h时对细胞的抑制作用最明显，与低浓度药物组比较，高浓度药物组对细胞的抑制作用增强（P〈0．05）；实验组细胞凋亡率分别为（3．22±0．25）％、（5．29±0．27）％，与对照组（0．75±0．67）％和错义寡核苷酸组（1．46±0．60）％比较，差异有统计学意义（P〈0．05）；实验组G1期细胞分布率分别为（53．31±1．30）％、（59．98±0.95）％，与对照组（47．73±1．18）％和错义寡核苷酸组（49．48±1．09）％比较，差异有统计学意义（P〈0．05）；实验组PDGF—α受体在SRA01／04中表达下调。结论PDGF-α受体沉默能抑制人晶状体上皮细胞的增殖，诱导其凋亡。
Objective To investigate the effect of PDGF-α receptor silence on proliferation and apoptosis of human lens epithelial cell in vitro, and to provide the treatment method for posterior capsule opacification. Methods Lens epithelial cells were cultured in vitro and PDGFR-α receptor antisense oligonucleotides was transfected into cells by cationic liposomes, cell proliferation was detected by MTT experiment, cell apoptosis by Hoechst 33258, cell cycle and apoptosis by Flow Cytometer, and gene expression by one step Reverse transcription Polymerase Chain Response （RT-PCR）. Results PDGF-α receptor antisense oligonucleotides had effect on SRA01/04 cells 24h-72h, and among all of them SRA01/04 cell 72h expressed most significant inhibitory effects, compared with the low concentration of the drug group, the high concentration of the drug group had stronger effect on inhabitation （P 〈0.05）; the SRA01/04 cells apoptosis ratio of experimental group were （3.22±0.25）% and （5.29±0.27）%, showing significance with control group （0.75±0.67）% and missense oligonueleotide group （1.46±0.60）% （P 〈0.05）; percentage of ceils in G1 phase in experimental group were （53.31±1.30）% and （59.98±0.95）%, showing significance with control group （47.73±1.18）% and missense oligonucleotide group （49.48±1.09）% （P 〈0.05）; but the experimental group of PDGF-α receptor had weak expression in SRA01/04. Conclusions PDGF-α receptor silence can inhibit human lens epithelial cell proliferation and induce apoptosis.
出处
《中国实用眼科杂志》
CSCD
北大核心
2012年第11期1377-1380,共4页
Chinese Journal of Practical Ophthalmology
