摘要
目的为肝癌的治疗提供新思路。方法将对数生长期肝癌Hep1细胞随机分为6组,空白对照组仅加入培养液,实验对照组加入等量溶媒二甲基亚砜(DMSO),姜黄素1～4组分别加入姜黄素5、10、15、20μmol/L。各组均置于常氧环境中培养24 h。RT-PCR法检测HIF-1αmRNA表达,Western blot法检测HIF-1α蛋白表达。结果姜黄素2～4组HIF-1αmRNA表达均明显高于空白对照组(P均<0.05)。姜黄素1～4组HIF-1α蛋白表达均明显高于空白对照组(P均<0.01)。结论常氧条件下,姜黄素呈浓度依赖性方式上调肝癌Hep1细胞HIF-1α的表达。
Objective To provide new ideas for the treatment of hepatocellular carcinoma.Methods Hepatocellular carcinoma Hep1 cells in logarithmic growth were randomly divided into 6 groups,the blank control group was treated with nutrient solution,the experimental control group was treated with equal amount of dimethyl sulphoxide,curcumin 1-4 groups were treated with curcumin of 5,10,15,20 μmol / L.All the groups were cultured under normal oxygen conditions for 24 hours.HIF-1α mRNA expression was measured by RT-PCR,and HIF-1α protein expression was measured by Western blot.Results HIF-1α mRNA expression in curcumin 2-4 groups were all higher than that in blank control group（all P 0.05）.HIF-1α protein expression in curcumin 1-4 groups were all higher than that in blank control group（all P0.01）.Conclusion Under normal oxygen conditions,curcumin can increase HIF-1α expression in hepatocellular carcinoma Hep1 cell in concentration-dependent manner.
出处
《山东医药》
CAS
北大核心
2010年第43期13-15,共3页
Shandong Medical Journal
基金
2008年广西壮族自治区研究生教育创新计划项目(桂学位[2008]14号556)
关键词
肝癌Hep1细胞
姜黄素
缺氧诱导因子-1Α
常氧条件
hepatocellular carcinoma Hep1 cell
curcumin
hypoxia-inducible factor-1α
normal oxygen conditions
