摘要
目的:研究表皮生长因子对人晶状体上皮细胞迁移的影响。方法:DMEM+100g/LFBS将人晶状体上皮细胞培养在T-25培养瓶内,划痕法检测EGF的促细胞迁移作用,蛋白质免疫印迹(Westernblot)方法检测β-catenin蛋白表达。结果:人晶状体上皮细胞在20μg/LEGF作用下,其迁移距离随着作用时间的延长而增加,在48h达到高峰;人晶状体上皮细胞在不同浓度EGF作用48h后,其迁移距离随着EGF浓度的增加而增加,在20μg/L时达到高峰;人晶状体上皮细胞在10μg/LEGF作用下,β-catenin蛋白表达水平随着作用时间的延长而升高,在2h达到高峰;人晶状体上皮细胞在不同浓度EGF作用2h,β-catenin蛋白表达水平随着EGF浓度的增加而升高,在10μg/LEGF作用下达到最大值。结论:EGF对人晶状体上皮细胞具有促进其迁移的作用,β-catenin在晶状体上皮细胞中的表达趋势说明其在晶状体上皮细胞迁移过程中起重要的作用。
AIM： To study the effect of epidermal growth factor （EGF） on the transfer of human lens epithelial cells.
METHODS： Human lens epithelial cells were cultured in DMEM＋100g/L FBS in the culture bottle. The scratch wound assay was used for examining the effect of EGF to human lens epithelial cells in their transference. Western blot method was performed to determine the expression of β -catenin protein.
RESULTS： At the concentration of 20μg/L EGF treatment, the transferring distance of human lens epithelial cells increased with the treating time lasting, reached the peak after 48h treatment. After the 48h EGF treatment, the transferring distance of human lens epithelial cells increased with treatment concentration increasing, with the peak at the concentration of 20μg/L. Further more, at the concentration of 10μ g/L EGF treatment, the expression of β-catenin protein of human lens epithelial cells increased with the treatment time lasting, with the peak after 2h treatment. After the 2h EGF treatment, the expres- sion of β-catenin protein of human lens epithelial cells increased with the treatment concentration increasing, with the peak at the concentration of 10μg/L.
CONCLUSION： EGF can promote the transferring of human lens epithelial cells. The expression pattern of βcatenin protein proves that β-catenin has important effect on the transference of human lens epithelial cells.
出处
《国际眼科杂志》
CAS
2006年第1期68-71,共4页
International Eye Science
