摘要
目的:观察降糖消脂片对2型糖尿病(T2DM)模型大鼠的降糖降脂作用,为临床治疗T2DM提供实验依据。方法:采用高脂高糖饲料喂饲大鼠4周,诱发胰岛素抵抗(IR),再按低剂量链脲佐菌素(streptozotocin,STZ)25 mg.kg-1ip,诱发高血糖症,造成T2DM大鼠模型。将实验动物分为正常对照组、模型组、降糖消脂片高、中、低(8,4,2 g.kg-1)剂量组、二甲双胍片(0.2 g.kg-1)组和金芪降糖片(2 g.kg-1)组,连续ig给药8周后杀检,测定空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、胰岛素(INS)、肿瘤坏死因子(TNF-α)及进行胰腺和肝脏的病理学检查。结果:大鼠在高脂高糖饮食4周时发生高胰岛素血症伴TC增高,成功诱发出IR。STZ ip后造成T2DM大鼠模型。口服8周后,与模型组比较,降糖消脂片高剂量组FBG明显降低(P<0.01),降糖率达到31.23%,INS水平显著增高(P<0.05),TG明显降低(P<0.01),中剂量组TG及TC明显降低(P<0.01～P<0.05),各剂量组TNF-α有降低的趋势;病理结果显示,与模型组比较,高剂量组胰岛数明显增多(P<0.05),中、低剂量组肝脂肪变性明显改善(P<0.05)。结论:降糖消脂片对T2DM模型大鼠有明显的降糖、降脂的作用。
Objective： To explore the effect of blood glucose and serum lipid in rats with type 2 diabetes of type 2 diabetic mellitus （T2DM）. Method： The rats insulin resistant. And a low dose streptozotocin （STZ） Jiangtang Xiaozhi （JTXZ） tablets on decreasing levels of and provide the experimental basis for the clinical therapy were fed on a diet enriched in fat and glucose to induce was injected intrapertoneally （25 mg·kg^（-1）） to induce hyperglycemia to establish T2DM. The experimental rats were divided into normal control group, model group, JTXZ tablets groups （8, 4 and 2 g ·kg^（-1））, metformin group （0.2 g ·kg^（-1）） and Jinqi Jiangtang tablets group （2 g^kg-＇）. After all the treatment groups were received their own medicine for 8 weeks, the rats were sacrificed and fasting blood glucose （FBG） , serum lipids （TC and TG） , insulin （INS） and tumor necrosis factor （TNF-a） were determined. A pathological examination was performed for the pancreas and the livers. Result： Hyperinsulinism with high level TC occured and the IR was successfully induced when the rats were fed on an enriched in fat and glucose diet for 4 weeks. Low dose of STZ were injected to establish T2DM. The FBG and TG in high dose of JTXZ tables group were significantly decreased （31.23% , P 〈0.01 ） and the INS were increased （P 〈0.05） comparing to the model rats. In the middle dose group the TG and TC were decreased significantly （P 〈0.01 -P 〈 0.05）. The TNF-a in the 3 dose groups showed a decreasing trend. The morphological study demonstrated that number of islets markedly increased in high dose group compared to the model group （P 〈 0.05）. The liver steatosis in middle and low dose groups was significantly milder than that in model group （P 〈 0.05 ）. Conclusion ： JTXZ tablets have obvious effects for decreasing levels of blood glucose and serum lipid in T2DM rats.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第21期161-165,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
综合性中药新药研究开发技术大平台(2009ZX09301-005-007)
重大新药创制项目(2010ZX09102-213)
关键词
降糖消脂片
2型糖尿病
链脲佐菌素
血糖
血脂
Jiangtang Xiaozhi tablets
type 2 diabetes mellitus
streptozotocin
blood glucose
serum lipid
