摘要
目的:探讨降糖消脂片改善胰岛素抵抗(insulin resistance,IR)及降脂的作用。方法:采用高脂饲料喂养Wistar大鼠1个月,建立IR大鼠模型。70只大鼠分为模型对照组、阳性药吡格咧酮组5 mg·kg-1、阳性药辛伐他汀组4 mg·kg-1、降糖消脂片剂量分别为生药8,4,2 g·kg-1,10只/组,连续灌胃给药(ig)2个月,同时继续高脂饲料喂养,普通饲料组为正常对照组。分批进行高胰岛素-正葡萄糖钳夹实验(正糖钳实验)。实验结束后,取血测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、超氧化物歧化酶(SOD)、丙二醛(MDA)、游离脂肪酸(FFA)等指标。取肝和胰腺称重,计算脏器指数,并作病理切片,观察其形态变化。结果:模型组葡萄糖输注率(GIR)明显低于正常组,显示模型组大鼠存在明显的IR;降糖消脂片高、中剂量组的GIR比模型组明显增加,IR明显改善;降糖消脂片各剂量组TG下降极其明显;高剂量组TC比模型组明显降低、HDL-C明显增高、LDL-C明显降低;降糖消脂片各剂量组SOD明显升高、MDA明显降低;高剂量组肝脏指数与模型组比较有所下降。降糖消脂片各剂量组肝脏、胰腺的病理变化有不同程度的减轻。结论:降糖消脂片具有明显的改善IR及降脂作用。
Objective： To explore effects of Jiangtang Xiaozhi （JTXZ） tablets for improving insulin resistance and decreasing blood lipids. Method： Wistar rat model with insulin resistance was established by taken high fat feed for one month. Thereafter, seventy rats were randomly and equally assigned to 7 groups （10 rats/group）, including a normal control group with common-feed, a model group with high fat feed and without any treatment, two positive control groups by treatment of Pioglitazone Hydrochloride tablets（5 mg·kg-1）and simvastatin tablets （4 mg·kg-1）, and three JTXZ tablets groups at dose of 8,4, 2 g·kg-1,by received intragastric administration daily for 2 months concurrently continued to take high fat feed. Total cholesterol （TC）, triglyceride （TG）, high density lipoprotein-cholesterol（HDL-C）, low density lipoprotein-cholesterol（LDL-C）, super oxide dismutase（SOD）, malonic dialdehyde（MDA） and free fatty acids（FFA） were measured following glucose infusion rate （GIR） and euglycemic clamp test. The liver and pancreas were weighted to calculate the viscera index and performed pathological slice up for observation of morphological change. Result： GIR was significantly decreased in model group comparing to normal control group and indicating an obvious IR in model group. In comparison with model group, GIR was significantly increased in JTXZ tablets groups at dose of 8, 4 g·kg-1 and indicating a noted improvement of IR; serum TC levels were markedly decreased in JTXZ tablets group at dose of 8 g·kg-1; serum TG levels were also significantly reduced in JTXZ tablets groups with all doses; serum HDL-C levels was evidently enhanced and serum LDL-C levels was significantly reduced in JTXZ tablets group at dose of 8 g·kg-1; SOD activities were markedly increased and MDA were notedly decreased in JTXZ tablets groups at all doses; the liver index was reduced in JTXZ tablets group at dose of 8 g·kg-1. The pathological observation indicated morphological improvement of liver and pancreas at various degrees in JTXZ tablets groups at all doses compared with model group. Conclusion： JTXZ tablets have obvious effects on improving insulin resistance and decreasing blood lipids.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第24期218-222,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
科技部重大新药创制项目(2009ZX09303-003)
科技部重大新药创制项目(2010ZX09102-213)
