摘要
目的:观察高浓度葡萄糖条件对体外培养人视网膜色素上皮hRPE细胞上CD44表达的影响。方法:用MTT方法检测在正常浓度葡萄糖5.6mmol/L和高浓度葡萄糖30.0mol/L作用(24h、48h、72h)RPE细胞增生的影响,并用流式细胞仪法检测两种浓度葡萄糖作用人RPE细胞表面CD44的表达强度。结果:在高浓度葡萄糖30.0mol/L的作用下,人RPE细胞增殖受到抑制,较正常浓度葡萄糖5.6mmol/L下24h、48h、72h的抑制率分别为11.40%、6.20%、5.36%。正常浓度葡萄糖培养下的人RPE细胞表面只有极少量CD44的表达,30.0mmol/L葡萄糖培养下的RPE细胞表面CD44的表达量为40.39%,两组比较差异有统计学意义(P<0.05)。结论:高浓度葡萄糖可以抑制体外培养的人RPE细胞的增殖,高糖可诱导人RPE细胞表面的CD44的表达增强。
Objective. To investigate the influence of high glucose on expression of CD44 in human reti- nal pigment epithelial （hRPE） cells in vitro. Methods. Proliferation of hRPE cells cultured with 5.6mmol/ L and 30.0 mmol/L of glucose for 24, 48and 72h were measured by MTT colorimetry. Expression level of CD44 in hRPE cells with 5.6 mmol/L and 30.0 mmol/L of glucose were measured by flow cytometer. Resuit. The proliferative inhibition on the surface of hRPE cells cultured with 30.0 mmol/L of glucose for 24, 48 and 72h were 11.4%,6.20% ,5.36%, respectively comparing with that cultured with 5.6 mmol/L of glucose. The expression of CD44 in RPE cells cultured with 30.0 mmol/L glucose was 40.39%, while only a little quantity of CD44 expression was detected in REP cells cultured in 5.6 mmol/L showing signif- icant difference between the two groups （P〈0.05）. Conclusions. High glucose can inhibit the prolifera-tion of RPE cell in vitro, while increase the expression of CD44.
出处
《海南医学院学报》
CAS
2012年第10期1388-1390,共3页
Journal of Hainan Medical University
基金
中国高校医学期刊临床专项资金项目(112210338)~~
