摘要
采用免疫组化染色研究正常人皮肤的CD44表达情况，发现CD44有选择性地存在于皮肤的不同部位。表皮中基底细胞及棘细胞表面有CD44，而颗粒层及用质层均无CD44。真皮组织无CD44，而毛囊外根鞘，皮脂腺毛囊口及外分泌汗腺均有CD44表达。测定了银屑病患者表皮的CD44，结果是损害处表皮的棘细胞及基底细胞表面CD44比无损害和治愈后的表皮要显著得多。用逆转录聚合酶链反应（RT－PCR）分析CD44的类型，结果为CD44的标准型。
The distribution of CD44 in human skin was studied with immunohistochemical staining. CD44 displayed a selective expression in. various location of the skin. It has been present on the surface of hasal and spinous cells but absent in the granular and cornified cell layers of epidermis, being also absent in the dermis.The level of CDM expression in psoriatic epidermis was assessed and overexpression of CD44 was observed in involved proliferative epidermis as compared with uninvolved and cured epidermis. Several CD44 isoforms based on analysis by reversetranscriptase polymerase chain reaction (RT-PCR). appeared to be present in the psoriatic lesion at the transcriptional level.
出处
《空军总医院学报》
1995年第2期72-74,共3页
Journal of General Hospital of Air Force,PLA
关键词
牛皮癣
聚合酶链反应
免疫组织化学
CD44
CD_(44) Immunohistochemistry Psoriasis Polymerase chain reaction Reverse transcription
