摘要
目的:研究海藻萃取物糖康乐在高糖条件下对兔视网膜色素上皮(RPE)细胞超氧化物歧化酶(SOD)表达的影响。方法:应用体外培养的兔RPE细胞,分成空白对照组、高糖组(用25mmol/L)葡萄糖处理、糖康乐组和牛磺酸组4组(n=6),应用高浓度葡萄糖后,糖康乐组加以不同浓度的糖康乐,同时应用牛磺酸作为阳性对照,测定不同浓度的糖康乐对RPE细胞SOD表达的影响。结果:在高糖条件下,高糖组RPE细胞SOD含量明显降低(2024±91→747±353kat/L)(t=8.570,P<0.001),糖康乐在浓度为0.002mg/L(t=3.207,P<0.01)、0.02mg/L(t=4.235,P<0.01)和0.2mg/L(t=3.748,P<0.01)的时候可以显著地抑制SOD表达的降低,与高糖组相比较有显著的差异,与阳性对照组的牛磺酸组相比,未见明显差异。结论:糖康乐可保护高糖导致的RPE细胞SOD表达的降低。
AIM： To investigate the effect of Tangkangle, the compound which was derived from algae extracts, on expression of superoxide dismutase （SOD） in cultured rabbit retinal pigment epithelial （RPE） cells under hyperglycemia condition. METHODS： We used rabbit RPE cells in vitro and divided them into 4 groups： blank control group, high glucose group dealt with 25mmol/L glucose, Tangkangle group and taurine group （n = 6）. After glucose of high concentration was added to RPE cells in vitro, Tangkangle group was added Tangkangle of different concentrations. In the meantime, taurine group was disposed with taurine as positive control. Effects of Tangkangle of different concentrations were determined on the expression of SOD in cultured RPE cells. RESULTS： Under hyperglycemia condition, the content of SOD in high glucose group decreased remarkably （ t = 8. 570, P〈 0.001 ）. Tangkangle exhibited significant inhibition on reduction of SOD expression at the concentrations of 0. 002mg/L （t=3.207, P〈0.01）, 0.02mg/L （t=4.235, P 〈0.01） and 0.2mg/L （t=3.748, P〈0.01）, and there was significant difference compared with high glucose group. The activity of Tangkangle was comparable to that of taurine （t=3.530, P〈0.01）. CONCLUSION： Tangkangle of certain concentrations could protect RPE cells from anti-oxidative damage by inhibiting the decrease of SOD expression.
出处
《国际眼科杂志》
CAS
2007年第6期1584-1586,共3页
International Eye Science
关键词
糖康乐
超氧化物歧化酶
视网膜色素上皮细胞
高糖
Tangkangle
superoxide dismutase
retinal pigment epithelial cell
hyperglycemia
