摘要
目的:建立活血定痛酊的质量标准。方法:采用薄层色谱(TLC)法对方中川芎、骨碎补、马钱子进行定性鉴别;采用高效液相色谱法对制剂中乌头碱和阿魏酸进行含量测定。结果:TLC斑点清晰、分离度好、专属性强;乌头碱的进样量在0.18～1.08μg(r=0.9999)范围内与峰面积积分值呈良好线性关系,平均回收率为97.17%,RSD=1.31%(n=9);阿魏酸检测浓度在5.2～15.7μg.mL-1(r=0.9998)范围内与峰面积积分值呈良好线性关系,平均回收率为97.51%,RSD=1.40%(n=9)。结论:所建标准可用于活血定痛酊的质量控制。
OBJECTIVE：To establish the quality standard of Huoxue dingtong tincture.METHODS：TLC method was used for the identification of Ligusticum chuanxiong,Drynaria fortunei and Strychnos nuxvomica;HPLC method was adopted to determine the contents of aconitine and ferulic acid.RESULTS：TLC spots were clear,well-separated and specific.The linear range of aconitine was 0.18～1.08 μg（r=0.999 9）with an average recovery of 97.17%（RSD=1.31%,n=9）.The linear range of ferulic acid was 5.2～15.7 μg.mL-1（r=0.999 8）with an average recovery of 97.51%（RSD=1.40%,n=9）.CONCLUSION：The method can be used for the quality control of Huoxue dingtong tincture.
出处
《中国药房》
CAS
CSCD
2012年第39期3703-3706,共4页
China Pharmacy
基金
全军医药卫生科研基金课题(2006071001)
关键词
活血定痛酊
川芎
骨碎补
马钱子
乌头碱
阿魏酸
Huoxue dingtong tincture
Ligusticum chuanxiong
Drynaria fortunei
Strychnos nuxvomica
Aconitine
Ferulicacid
