摘要
目的:研究中药在左氧氟沙星对金黄色葡萄球菌耐药突变选择窗(MSW)上的影响。方法:采用琼脂平板稀释法测定几种中药制剂的最低抑菌浓度(MIC)、防突变浓度(MPC),得出其选择指数(SI)。选择SI值较低的中药制剂双黄连与大蒜素分别与左氧氟沙星联用,琼脂平板稀释法测定不同浓度下双黄连/大蒜素与左氧氟沙星联用对金黄色葡萄球菌MSW上的影响。结果:双黄连和左氧氟沙星联用(2MIC+8MIC,4MIC+4MIC)可使左氧氟沙星对金黄色葡萄球菌的MSW缩小至0.4～0.8倍;而不同浓度大蒜素与左氧氟沙星联用,则未见明显作用。结论:双黄连注射剂与左氧氟沙星联用可缩小左氧氟沙星对金黄色葡萄球菌的MSW,从而降低金黄色葡萄球菌对左氧氟沙星的耐药性。
OBJECTIVE：To study the effects of traditional Chinese medicine（TCM）on mutant selection window（MSW）of staphylococcus aureus ATCC29213 to levofloxacin.METHODS：By using agar plate dilution method,the MIC and mutant prevention concentration（MPC）of TCM preparations were determined to obtain SI.Shuanghuanlian or allitridi with low SI were combined with levofloxacin,and the effects of different concentrations of Shuanghuanlian or allitridi on MSW of Staphylococcus aureus ATCC29213 to levofloxacin were determined by using same method.RESULTS：Shuanghuanlian combined with levofloxacin（2MIC＋8MIC,4MIC＋4MIC）decreased MSW of Staphylococcus aureus ATCC29213 to levofloxacin for 0.4～0.8 times.Otherwise,different concentrations of garlicin combined with levofloxacin had no obvious effect.CONCLUSION：Combinations of levofloxacin and shuanghuanglian injections can decrease MSW of Staphylococcus aureus ATCC29213 to levofloxacin so as to reduce antibiotic resistance.
出处
《中国药房》
CAS
CSCD
2012年第39期3659-3661,共3页
China Pharmacy
