摘要
考察茚三酮比色法测定灭菌乳中氨基酸条件的主要影响因素。通过单因素及正交试验对其测定条件进行改进。结果表明:水浴时间对测定结果影响显著,要严格控制。茚三酮比色法测定灭菌乳中氨基酸的最佳条件为:水浴时间30min、水浴温度70℃、茚三酮用量1.2mL,在此条件下测得灭菌乳中氨基酸含量为1.62g/100mL。
One-factor-at-a-time and orthogonal array design methods were used to optimize the major reaction conditions affecting the determination of amino acid content in sterilized milk by ninhydrin colorimetric method.Water-bath heating time influenced significantly determination results so that it required strict control.The optimal determination conditions were 1.2 mL of ninhydrin solution and heating for 30 min in 70 ℃ water-bath.Under these conditions,the amino acid content in sterilized milk was determined to be 1.62 g/100 mL.
出处
《乳业科学与技术》
2012年第2期38-40,共3页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金
鞍山师范学院院级课程建设项目(2010kcjs23)
关键词
灭菌乳
氨基酸
测定条件
sterilized milk
amino acid
determination conditions
