摘要
目的 实验研究溴氰菊酯 (DM)和氯菊酯 (PM)对大鼠脑组织及肝脏某些生物转化酶的影响。方法 应用荧光分光光度法、二硝基苯肼比色法和Habig法检测 7 乙氧基试卤灵 O 脱乙基酶(EROD)、B型单胺氧化酶 (MAOB)和谷胱甘肽S 转移酶 (GST)活力。结果 整体实验中 ,DM染毒动物肝脏EROD、MAOB活力分别下降了 47.94%和 2 2 .76 % ;脑组织和肝脏GST活力分别增加了 2 2 .2 0 %和39.5 5 % ;多氯联苯 (PCB)的诱导EROD效应被明显抑制。PM染毒动物肝脏MAOB活力下降了36 .11% ;脑组织和肝脏GST活力分别增加了 36 .76 %和 39.2 5 %。体外实验中 ,DM在 1× 10 -4 ～1× 10 -3 mol/L、PM在 1× 10 -5～ 1× 10 -3 mol/L的浓度时 ,肝微粒体EROD活力均受抑制 ,并有剂量 -效应关系 (r =- 0 .90、r =- 0 .84,P <0 .0 1) ;DM在 1× 10 -6～ 1× 10 -5mol/L的浓度时 ,脑线粒体MAOB活力均受抑制 (r=- 0 .88) ;PM在 1× 10 -4 ～ 1× 10 -3 mol/L、DM在 1× 10 -5～ 1× 10 -3 mol/L的浓度时 ,脑组织胞浆GST活力均增强 ,与对照组的差异均有显著性 (r=0 .86、r =0 .83,P <0 .0 5 ,P <0 .0 1)。
Objective The effects of two pyrethroids,permethrin(PM) and deltamethrin(DM),on metabolizing enzymes in rat brain and liver were investigated.Methods Fluorescence spectrophotometry was applied to measure the activity of 7 ethoxyresorufin O deethylase(EROD);the activity of monoamine oxidase type B(MAOB) was measured with dinitrophenylhydrazine spectrophotometry;Habig's method was used to detect the glutathione S transferase(GST) activity.Results In vivo,DM could inhibit hepatic EROD and MAOB activities by 47.94% and 22.76% respectively and enhance the activities of GST in brain and liver by 22.20% and 39.55% respectively.Polychlorobiphenyl inducing effect was obviously inhibited too.PM could inhibit hepatic MAOB by 36.11%,and enhance brain and liver GST by 36.76% and 39.25% respectively.In vitro,it was found that both pyrethroids could inhibit hepatic microsomal EROD activities and showed dose effect relationship( r =-0.90, r =-0.84 respectively, P <0.01).Both could enhance the activities of brain cytoplasmic GST.DM could also inhibit the activities of brain mitochondrial MAOB.Conclusion The toxic effects of pyrethroids may relate to their interference with some metabolizing enzymes in brain and liver.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2000年第4期216-219,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金!(39430 110)资助重点项目
关键词
拟除虫菊酯
EROD
MAOB
GST
生物转化酶
脑
肝脏
Pyrethroids
7-ethoxyresorufin-O-deethylase
Monoamine oxidase type B
Glutathione S-transferase
