摘要
目的 观察溴氰菊酯 (DM)对原代培养大鼠神经星形胶质细胞存活率及胞内游离钙离子浓度 ([Ca2 + ]i)的影响。方法 以锥虫蓝染料排斥试验检测细胞染DM后存活率的变化 ;以Fura 2 /AM为荧光指示剂 ,RF 5 30 1PC荧光分光光度计测定细胞内 [Ca2 + ]i的变化。结果 1× 10 -5mol/L组染毒DM 72h后细胞存活率下降为 91.9% ,与对照组的差异有显著性 (P <0 .0 5 ) ,1× 10 -4mol/L组DM染毒4、12、4 8、72h后细胞存活率分别为 89.0 %、84 .8%、81.2 %和 79.2 % ,差异有显著性 (P <0 .0 1) ;1× 10 -7、1× 10 -6、1× 10 -5mol/L组DM染毒 5min后胞内 [Ca2 + ]i分别上升至 (45 1.4± 4 2 .3)、(5 36 .9± 4 7.5 )、(870 .9± 10 0 .5 )nmol/L ,与染毒前及对照组比 ,差异有显著性 (P <0 .0 1)。以后各染毒组胞内 [Ca2 + ]i逐渐下降 ,15min时 1× 10 -8、1× 10 -7、1× 10 -6、1× 10 -5mol/L染毒组胞内 [Ca2 + ]i分别降至 (12 4 .3± 6 .0 )、(131.3± 19.1)、(118.9± 1.4 )、(136 .6± 3.8)nmol/L ,与染毒前及对照组比差异均有显著性 (P <0 .0 5或P <0 .0 1)。结论 DM在体外可降低神经胶质细胞的存活率并短时升高胞内 [Ca2 + ]i。
Objective To study the effects of deltamethrin(DM) on cell survival rate and intracellular Ca 2+ (i) concentration in primary cultured astrocytes of rat. Methods The cell survival rate was measured by Typan Blue assay;the intracellular i concentration was determined by the fluorescent Ca 2+ indicator Fura-2/AM. Results The survival rate of astrocytes was decreased to 91.9% after astrocytes were incubated with 1×10 -5 mol/L DM for 72 h( P <0.05).The cell survival rates were 89.0%,84.8%,81.2% and 79.2% respectively when astrocytes were administered with 1×10 -4 mol/L DM for 4,12,24 and 72 h,which were remarkably lower than control groups( P <0.01).Comparing with controls and before DM treatment,sharp increases in i concentration[(451.4±42.3),(536.9±47.5) and (870.9±100.5) nmol/L respectively] were observed when astrocytes were incubated with 1×10 -7 ,1×10 -6 and 1×10 -5 mol/L DM for 5 minutes( P <0.01).After astrocytes were treated with 1×10 -8 ,1×10 -7 ,1×10 -6 ,1×10 -5 mol/L DM for 15 minutes,the i concentrations were decreased to (124.3±6.0),(131.3±19.1),(118.9±1.4), (136.6 ±3.8) nmol/L respectively,which were significantly different from those of controls and before treatment.And this situation was almost keeping stable to 30 min. Conclusion The cell survival rate was decreased and the i concentration was temporarily increased when astrocytes were treated with DM.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2003年第3期194-196,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
