摘要
作者前期对氰戊菊酯经Tn细胞代谢后结构变化的研究表明,氰戊菊酯的苯氧基被羟基所取代,α-氰基被氢基取代。为了验证氰戊菊酯经Tn代谢后毒性是否发生了改变,作者利用点滴法测定了氰戊菊酯及其经P450s修饰后的代谢产物对果蝇的毒性作用。结果显示氰戊菊酯对果蝇的半数致死量(LC_(50))为14.062μg·mL^(-1),而最大浓度的代谢产物处理果蝇未见其毒性。研究表明氰戊菊酯在体内经P450s代谢后的产物失去了对昆虫细胞的毒害作用,这一作用也可能是昆虫抗性产生的机制之一。
Our previous research showed that the structure of fenvalerate was modified after metabolism in Trichoplusia ni（Tn） cell. The cyano was lost and the phenoxyl group was substituted by the hydroxyl.In order to validate the toxicity change of the fenvalerate after the metabolizing process in Tn cells,topical application was used to measure toxic effects of fenvalerate and its derivative to Drosophila melanogaster.The median lethal concentration（LC_（50）） of fenvalerate was 14.062μg·mL^（-1）.Furthermore,its derivative has no toxicity.These data can explain that mixed-function oxideses are responsible for based resistance to insecticide.
出处
《上海交通大学学报（农业科学版）》
2010年第6期538-540,共3页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海自然基金项目(082R1410900)
上海市农科项目(SHNK 412)
关键词
果蝇
氰戊菊酯
点滴法
毒性
半数致死浓度
Drosophila melanogaster
fenvalerate
topical application
toxicity
median lethal concentration（LC_（50））
