摘要
目的:观察比较活骨Ⅱ方配伍不同引经药对股骨头坏死(ONFH)兔股骨头内血管内皮生长因子(VEGF)表达的影响,探讨其防治ONFH的作用机制。方法:84只兔经液氮冷冻法建立股骨头坏死模型后,随机分为以下6组:模型组活骨Ⅱ方组、活骨Ⅱ方加不同引经药组(牛膝、独活、细辛和桔梗),另14只设为伪手术组。造模后中药组立即分别给予活骨Ⅱ方(6 g·kg-1·d-1)、活骨Ⅱ方(6 g·kg-1·d-1)加引经药(牛膝0.75 g·kg-1·d-1,细辛0.15 g·kg-1·d-1,独活0.75 g·kg-1·d-1,桔梗0.75 g·kg-1·d-1,分别相当于临床等效量)灌胃,连续4周;模型和伪手术组给予生理盐水灌胃,所有动物均皮下注射重组人粒细胞集落刺激因子(rhG-CSF)30μg·kg-1·d-1,连续7 d。实验2周和4周时取左侧股骨头,分别行苏木精-伊红(HE)染色观察其组织病理学改变和墨汁灌注染色观察股骨头内血管形态变化,并经免疫组织化学法检测股骨头内VEGF的表达水平。结果:与伪手术组相比,模型组实验2周和4周时股骨头空骨陷窝率显著增多,血管面积及VEGF表达明显减少(P<0.01或P<0.05)。活骨Ⅱ方能显著降低股骨头空骨陷窝率,并提高血管面积及VEGF的表达(P<0.01或P<0.05)。与活骨Ⅱ组相比,牛膝组4周时空骨陷窝率显著减少,血管面积明显增多(P<0.01或P<0.05),2周和4周VEGF表达均明显增多(P<0.01);桔梗组4周时VEGF表达显著降低;细辛和独活组无显著变化。结论:引经药牛膝具有进一步提高活骨Ⅱ方上调股骨头内VEGF表达的作用,这可能是其有效改善股骨头血循环、防止坏死的机制之一。
Objective：To investigate the effect of Huogu Ⅱ formula and its combination with different meridian-guiding herb on the expression of vascular endothelial growth factor（VEGF） in the femoral head of rabbits with osteonecrosis of the femoral head（ONFH）,as well as to explore the mechanism of prevention and treatment for ONFH.Method： The animal model of ONFH was established by liquid nitrogen frozen in the rabbit left hind leg.Ninety-eight.Japanese white rabbits were randomized to sham-operated group,model group,Huogu Ⅱ group and Huogu Ⅱ plus achyranthes root,Pubescent angelica,asarum and Balloonflower respectively,evenly 14 in each group.During the course of ONFH animal model establishment,all the rabbits were subcutaneously injected with recombinant human granulocyte colony-stimulating factor（rhG-CSF）（30 μg·kg-1·d-1,for continuous 7 days）.Meanwhile normal saline and decoction of the formulae were administrated by gavage respectively.Materials were drawn at the second and forth week after model establishment Histopathological changes were observed by HE staining,vascular morphology was observed by ink perfusion,VEGF was measured by immunohistochemical assay in femoral heads of the left hind leg.Result： Compared with sham-operated group,the ratio of empty lacuna increased sharply,vessel area and VEGF levels decreased obviously in the model group.But in comparison with the model group,the empty lacuna ratio of Huogu II group decreased obviously,vessel area,VEGF increased significantly.Compared with Huogu II group,the empty lacuna ratio decreased and vessel area increased significantly at the forth week,and the expression of VEGF increased both at the second and forth week in the achyranthes root group.As for the balloonflower group,empty lacuna ratio showed the increase potention trend,and the number of VEGF-positive cells decreased obvious at the forth week.There were no significant changes in Asarum and Pubescent angelica combination group.Conclusion： The medicinal guide achyranthes root could enhance the effect of Huogu Ⅱ formula to increase the expression of VEGF in the ONFH animal model.It may contribute to the preventive and therapeutic effect for ONFH.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期237-241,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金面上项目(30672770)
关键词
股骨头坏死
血管内皮生长因子
活骨Ⅱ方
引经
osteonecrosis of the femoral head
vascular endothelial growth factor
Huogu Ⅱ formula
meridian-guiding herb
