摘要
目的观察急性心肌梗死(AMI)患者外周血干细胞和干细胞因子(stem cell factor,SCF)的变化及两者的相关性。方法AMI患者共25例,收集患者入院即刻,第13、、71、4和28天的抗凝血各2 ml,用流式细胞仪检测如下细胞表面标记物:CD34+、血管内皮细胞生长因子受体-2(VEGFR-2+)、CD31+、CD144+、CD133+、CD45+,比较各时间点的差异。同时用酶联免疫吸附法检测血清SCF水平。结果AMI后CD34+细胞逐渐升高,到第7天时达到峰值,而VEGFR-2+、CD31+、CD144+、CD133+细胞则出现下降趋势,并且于第7天时达到谷值(P<0.05)。AMI患者外周血清SCF浓度随时间推移呈下降趋势(P<0.01),但AMI后第1天时SCF浓度与外周血CD34+细胞数呈直线相关(P<0.01)。结论AMI可引起外周血干细胞数量增多以及血清SCF浓度升高,但也许会消耗循环中有内皮功能或向内皮分化的细胞。这些干细胞数量的增多可能受SCF浓度的调节。
Objective To observe the changes of peripheral blood stem cells and stem cell factor（SCF） in patients with acute myocardial infarction（AMI） and the relationship between AMI and stem cells and SCF in peripheral blood. Methods Blood samples （2 ml） were collected from 25 AMI patients on admission, 1,3,7,14 and 28 days, and were examined with flow cytometry and ELISA. Results Flow cytometry revealed that the number of CD34 ^＋ cells was higher on d 7 of AMI than on admission （ P 〈 0.05）, but VEGFR-2 ^＋ , CD31 ^＋ , CD144^＋ ,CD133^＋ positive cells were less on d 7 of AMI than on admission （ P 〈 0.05）. ELISA results showed that serum SCF value was highest on admission, and decreased with the time. CD34^＋ cells had the linear correlation with SCF on d 1 after AMI（ P 〈 0.01）. Conclusion Circulating stem cells in peripheral blood can be mobilized and serum SCF would increase in AMI patients, but some circulating endothelial progenitor cells would be consumed. The increase in the stem cells may be regulated by the SCF in vivo.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2007年第2期82-85,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
关键词
心肌梗塞
干细胞
干细胞因子
流式细胞术
myocardial infarction
stem cells
stem cell factor
flow cytometry
