摘要
目的:建立测定双黄连制剂中连翘苷含量的方法。方法:采用高效液相色谱法。直接稀释法制备供试品溶液,色谱柱为AgilentZorbaxSB-C18柱,柱温为35℃,流动相为乙腈-0.012mol.L-1醋酸钠溶液(v/v=23:77),流速为1mL.min-1,检测波长为277nm,进样量为10μL。结果:连翘苷进样量在0.11～5.5μg范围内与峰面积积分值呈良好的线性关系(r=0.9999),连翘苷平均回收率为98.8%,RSD=0.3%(n=6)。结论:改进后的方法简便、高效、准确、重复性好,可用于双黄连制剂的质量控制。
OBJECTIVE：To establish a simple and efficient method for the content determination of forsythin in the Shuanghuanglian preparations.METHODS：HPLC method was adopted.Test solution was prepared by direct dilution.The analysis was carried out on an Agilent Zorbax SB-C18 column using a mixture of acetonitrile-0.012 mol·L-1 sodium acetate solution（v/v=23：77） as mobile phase at a flow rate of 1 mL·min-1.The column temperature was 35 ℃,the detection wavelength was set at 277 nm and the injection volume was 10 μL.RESULTS：The linear range of forsythin was 0.11～5.5 μg（r=0.999 9）with an average recovery of 98.8%（RSD=0.3%,n=6）.CONCLUSIONS：The method is simple,effective,accurate and reproducible,and it is suitable for the quality control of Shuanghuanglian preparations.
出处
《中国药房》
CAS
CSCD
2012年第16期1529-1531,共3页
China Pharmacy
