摘要
目的建立Beagle犬血浆样品中莫西沙星HPLC测定方法,研究Beagle犬口服盐酸莫西沙星片后体内药动学。方法色谱柱:依利特C18色谱柱(4.6mm×250mm,5μm);流动相:乙腈-0.05mol/L磷酸盐缓冲液(含0.01mol/L四丁基硫酸氢铵,pH=3.8)=15:85;检测波长:295nm;柱温:25℃;流速:1.0mL/min;进样量:20μL。Beagle犬口服盐酸莫西沙星片后定时取血,HPLC法测定血浆药物浓度,DAS2.0软件计算药动学参数。结果莫西沙星在Beagle犬体内的药-时曲线符合二室开放模型,主要药动学参数Tmax为(3.33±0.23)h,Cmax为(7.35±0.40)μg/mL,AUC为(118.37±13.05)μg/mL.h,t1/2α为(3.64±0.34)h,t1/2β为(13.49±0.50)h。结论此测定方法操作简单,专属性强,重现性好,所得药动学参数有助于对莫西沙星的进一步研究。
Objiective To establish a high performance liquid chromatographic （HPLC） method for the measurement of moxifloxacin in the plasma of Beagle dogs, and to investigate the pharmacokinetics of moxifloxacin hydrochloride tablet in Beagle dogs. Method The analysis was performed on a Elite C18 column （4.6mm~×250 mm, 5μm） at 25℃ with the mobile phase of acetonitrile-water （0.05mol/L buffer phosphate with 0.01mol/L tetrabutylammonium hydrogen sulfate, pH=3.8） 15：85 at a flow rate of 1.0mL/min, the sample size was 20μL and the detect wavelength was 295nm. The concentrations of moxifloxacin in the plasma of Beagle dogs were determined by HPLC method and the pharmacokinetic parameters of moxifloxacin were calculated by DAS2.0 soft ware. Results Mean plasma concentration-time curve of moxifloxacin in Beagle dogs was in line with two-compartments model. Main pharmacokinetic parameters were as follows： Tmax： （3.33±0.23） h, Cmax （7.35±0.40） μg/mL, AUC ： （118.37±13.05） μg/mL h, t1/2α（3.64 ±0.34） h, tl/2β（13.49±0.50） h. Conclusion The methodis specificitive, reproducible and easy to process. The obtained pharmacokinetic parameters of moxifloxacin will be helpful for the further study of moxifloxacin.
出处
《国外医药（抗生素分册）》
CAS
2012年第2期87-90,共4页
World Notes on Antibiotics
基金
河北省卫生厅科学基金资助项目(编号:05093)
