摘要
目的制备伊达比星脂质体，建立伊达比星脂质体包封率的测定方法。方法采用薄膜分散．超声合并挤压法制备伊达比星脂质体，通过葡聚糖凝胶柱色谱分离游离药物和脂质体，用HPLCN定伊达比星含量，计算包封率。结果伊达比星脂质体平均包封率为65．42％，载药量为7．70％。凝胶柱色谱柱回收率99．76％，伊达比星在0．05-30．0μg／mL范围内线性关系良好（r=0．9999）。结论薄膜分散-超声合并挤压法适用于制备伊达比星脂质体，分析方法准确可靠可以用于伊达比星脂质体包封率的测定。
Objective To prepare idarubicin liposome and establish a method for determination of its entrapment efficiency. Methods The liposome was prepared by film dispersion-ultrasonic combined extrusion technique. The liposome and free idarubicin were separated by Sephadex-G50 chromatography. The concentration of idarubicin in the liposome was detected by HPLC, then the entrapment efficiency was evaluated. Results The mean entrapment efficiency of idarubicin liposome was 65.42% and the drug loading was 7.70%. The recovery rate was 99.76%. The standard curve was linear over the range of 0.05-30.0μg/mL with the correlation of 0.9999. Conclusion The film dispersion-ultrasonic combined extrusion technique is suitable for preparing idarubicin liposomes. This HPLC method was accurate and sensitive, and can be applied to the determination of idarubicin liposomes.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2014年第6期447-450,共4页
Chinese Journal of Antibiotics
关键词
伊达比星
薄膜分散超声合并挤压法
脂质体
包封率
Idarubicin
Film dispersion-ultrasonic combined extrusion technique
Liposome
Entrapment efficiency
