摘要
目的:明确脑得生片的化学物质基础。方法:采用高效液相-二极管阵列-质谱(HPLC-DAD-MSn)联用技术检测并鉴定其主要化学成分。色谱柱为Agilent Zorbax SB-C18(4.6 mm×250mm,i.d,5μm),流动相为0.05%甲酸-水和乙腈,流速为0.5 ml.min-1。结果:在脑得生片中共检测到43个化学成分,通过比对紫外吸收图谱、标准品ESI-MSn质谱图提供的分子量及结构式,并结合文献报道,推断鉴定出其中22个化合物,分别为羟基红花黄色素A、4'-O-葡萄糖基葛根素、3'-羟基葛根素、大豆苷元-8-C-芹菜糖基(1-6)葡萄糖苷、葛根素、6″-O-木糖基葛根素、6″-O-芹菜糖基葛根素、3'-甲氧基葛根素、3'-甲氧基-6″-O-木糖葛根素、大豆苷、5,7-三羟基异黄酮-7-糖苷、葛根苷A、三七皂苷R1、人参皂苷Re、人参皂苷Rg1、大豆苷元、鹰嘴豆素A、人参皂苷Rb1、人参皂苷Rc、人参皂苷Rb2、人参皂苷Rb3和人参皂苷Rd。结论:初步明确了脑得生片的化学物质基础,为进一步提高脑得生片的质量控制标准提供了科学数据。
Objective: To identify the major components of traditional Chinese medicine Naodesheng tablet. Methods: A HPLC-DAD-MSn based method was developed to analyze and identify the major components of Naodesheng tablet.Separation was performed on an Agilent Zorbax SB-C18 column(4.6 mm×250 mm,i.d,5 μm) with mobile phase consisting of water with 0.05% formic acid and acetonitrile as gradient eluent at the flow rate of 0.5 ml·min-1. Results: A total of 43 components were detected,among which 22 were identified by comparing their UV absorption profiles,the information of molecular weights,and structures provided by ESI-MSn with those of available standards and reference data,such as Safflor yellow A,4′-O-Glucosylpuerarin,3′-hydroxypuerarin,Genistein-8-C-apiosy(1-6) glucoside,Puerarin,6″-O-xylosylpuerarin,6″-O-apiosylpuerarin,3′-methoxypuruerarin,3′-methoxy-6″-o-xylosylpuerarin,Daidzin,Genistin,Pueroside A,Notoginsenoside R1,Ginsenoside Re,Ginsenoside Rg1,Daidzein,Biochanin A,Ginsenoside Rb1,Ginsenoside Rc,Ginsenoside Rb2,Ginsenoside Rb3,Ginsenoside Rd. Conclusion: The proposed method can identify the main components of Naodesheng tablet and provide information for the quality control of this medicine.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2012年第1期32-42,共11页
Journal of Zhejiang University(Medical Sciences)
基金
浙江省杰出青年团队(R2080693)
关键词
葛根/化学
红花/化学
川芎/化学
三七/化学
色谱法
高压液相
色谱法
质谱分析法
Pueraria lobata/chemistry
Carthamus tinctorius/chemistry
Ligusticum chuanxiong/chemistry
Panax notoginseng/chemistry
Chromatography,high pressure liquid
Chromatography
Mass spectrometry
Drugs,Chinese herbal
