摘要
目的:建立葛根总黄酮的高效毛细管电泳(HPCE)指纹图谱。方法:运行缓冲液为30mmol·L-1硼砂溶液,分离电压为13kV,进样时间为5s,检测波长为254nm,柱温为25℃。结果:从葛根总黄酮HPCE指纹图谱中标定了14个共有峰,其总峰面积达到93%以上。结论:所建立的指纹图谱准确、可靠,可用于评价和控制葛根总黄酮的质量。
OBJECTIVE：To establish the HPCE fingerprints of total flavonoids from Pueraria lobata.METHODS：HPCE method was used with 30 mmol·L^-1 borax as buffer.Separation voltage was 13 kV and sampling time was 5 second.The detection wavelength was set at 254 nm,and the column temperature was maintained at 25 ℃.RESULTS：14 common peaks of total flavonoids from P.lobata were successfully separated,and total areas of common peaks were above 93%.CONCLUSION：Established HPCE fingerprint of total flavonoids from P.lobata is accurate and reliable.It can be used for the quality evaluation and control of total flavonoids from P.lobata.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第39期3709-3711,共3页
China Pharmacy
基金
武汉市科学技术计划项目(2002600513406)
