摘要
建立了以 0 .0 2 %磷酸 乙腈为流动相 ,梯度洗脱反相高效液相色谱同时测定中药材三七中三七皂苷R1 、人参皂苷Rg1 、Rb1 和Rd 4种皂苷的新方法。R1 、Rg1 、Rb1 和Rd 4种皂苷的加样回收率分别为 89.5 4 %、90 0 8%、82 .82 %与 84 .4 6 % ;线性范围分别为 0 .2 4 4～ 6 .110、0 .82 0～ 2 0 .5 10、0 .396～ 9.890与 0 .2 6 0～ 6 .5 0 0 μg。测定了不同规格、部位和来源的三七药材里的 4种皂苷R1 、Rg1 、Rb1 和Rd。方法准确可靠 ,结果稳定 ,重现性好 。
A new method for the simultaneous quantitative determination of notoginsenoside R 1, ginsenoside Rg 1, Rb 1 and Rd in Panax notoginseng using reversed phase high perfromance liquid chromatography was developed. Gradient elution was used for the baseline separation of the four saponins in 25 mins. The optimum chromatographic conditions were: analytical column, Dalian Elite Hypersil C18 column (i.d. 4.6×250 mm, 5 μm ); column temperature, 25℃; mobile phase with gradient elution. A(80% acetonitrile 0.02%phosphate acid solution, V/V ), B (0.02% phosphate acid solution, V/V ), A: 0 min, 12%; 0～20 min, 12%～60%; 20～35 min, 60%; 35～37 min, 60%～90%; 37～45 min, 90%. Detection at ultraviolet 203 nm with 360 nm as reference; flow rate 1.0 mL/min. The recoveries of R 1, Rg 1, Rb 1 and Rd were 89.54%, 90.08%, 82.82%, 84.46%, respectively, linear ranges 0.244～6.110, 0.820～20.510, 0.396～9.890, 0.260～6.500 μg, respectively. The method had been successfully applied to determine the contents of saponins in different specifications and in different parts of Panax notoginseng from multi origins.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2003年第6期731-734,共4页
Chinese Journal of Analytical Chemistry
基金
国家"十五"重大科技攻关 (No .2 0 0 1BA70 1A0 1)
国家重点基础研究发展规划 (G19990 5 44 0 5 )
关键词
反相高效液相色谱法
同时测定
分析
三七
中药材
皂苷
Reversed phase high performance liquid chromatography, panax notoginseng, traditional Chinese medicine
