摘要
[方法]建立同时测定青海地区锁阳中没食子酸和原儿茶酸含量的高效液相色谱法。[方法]采用Eclipse XDB C18柱(250.0 mm×4.6 mm,5.0μm),以甲醇0、.1%的三氟乙酸为流动相,检测波长:280 nm,流速:1 ml/min。[结果]青海地区锁阳中没食子酸含量为0.25～0.35 mg/g,原儿茶素含量为0.016 0～0.016 4 mg/g。[结论]该方法稳定,结果准确,重现性好,可为控制该药材和饮片的内在质量提供参考依据.
[Objective]The aim was to determine gallic acid and protocatechuic acid content in Cynomorium songaricum Ru in Qinghai by HPLC.[Method] The chromatographic column of Eclipse XDB C18（150 mm×4.6 mm,5.0 μm）was used.The mobile phase consisted of methanol and water with 0.1%TFA and the flow rate was 1.0 ml/min.The detection wavelength was 280 nm.[Result]It was showed that the content of gallic acid and protocatechuic acid in Cynomorium songaricum Ru was 0.25-0.35,0.016 0-0.016 4 mg/g.[Conclusion]The menod was rapid and accurate,and provide reference for controlling internal quality of crude drug.
出处
《安徽农业科学》
CAS
2012年第6期3831-3832,共2页
Journal of Anhui Agricultural Sciences
关键词
HPLC
锁阳
没食子酸
原儿茶酸
HPLC
Cynomorium songaricum Ru
Gallic acid
Protocatechuic acid
