摘要
目的：建立反相高效液相色谱法同时测定青海地区野生线叶龙胆中獐牙菜苦苷、龙胆苦苷、獐芽菜苷、异荭草苷的含量。方法：Kromasil C18柱（4.6 mm×250 mm,5μm）;流动相乙腈（A）-0.1%磷酸水溶液（B）,梯度洗脱,洗脱条件0.00-27.00 min,10%-17%A;27.00-45.00 min,17%-33%A;45.00-45.01 min,33%-100%A;45.01-55.00 min,100%-100%A;体积流量1.0 mL.min-1,检测波长240 nm,柱温25℃。结果：獐牙菜苦苷、龙胆苦苷、獐芽菜苷和异荭草苷均达到基线分离,分别在2.12-10.6,2.46-12.3,2.47-12.4,0.309-1.54 g.L-1线性良好,r分别为0.999 4,0.999 7,0.999 2,0.999 5。结论：该方法快速、准确、重复性好,为该类药材的质量控制提供了理论依据。
Objective： To establish a RP-HPLC method for simultaneously determination of swertiamarin, gentiopicroside, sweroside, isoorientin in Gentiana lawrencei from Qinghai province. Method： The RP-HPLC method was used. Chromatographic column was the Kromasil C18 column （4. 6 mm×250 mm,5 μm）. The gradient elution solvent system was composed of acetonitrile（A） and （0. 1% H3PO4 ） water （B）. The ratio of acetonitrile was as follows： 0. 00-27.00 min, 10% -17% （A） ; 27.00-45.00 min, 17% - 33% （A） ; 45.01-55. 00 min, 100%-100% （A）. The detective wavelength was 240 nm; the flow rate was 1 mL· min^-1 ; column temperature was set at 25 ℃. Result： Swertiamarin, gentiopicroside, sweroside and isoorientin were base-isolated. The method had good linearity within the ranges of 2. 12-10. 6 mg· mL^-1 for swertiamarin （r =0. 999 4）, 2.46-12. 3 g· L-1 for gentiopicroside （r = 0. 999 7）, 2. 47-12.4 g · L-1 for sweroside （r = 0. 999 2） and 0. 309-1.54 mg · mL^-1 for isoorientin （r = 0. 999 5 ）. Conclusion： The method is rapid, precise and repeatable, can be applied to control the quality of G. lawrencei.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第22期2884-2886,共3页
China Journal of Chinese Materia Medica
基金
青海省科技厅重点攻关项目(2004-N-142)
