摘要
目的探讨丁型肝炎患者肝组织中HDAg与HBsAg,HBcAg和HBV DNA表达及相关性。方法应用免疫组化双重染色及连续切片技术和原位杂交,检测了79例丁型肝炎患者肝组织佃ADAg,HBsAg,HBcAg和HBV DNA表达,以52例乙型肝炎作对照。结果丁型肝炎HBsAg,HBcAg检出率为81％,71％,乙型肝炎为94％,92％,两组比较有显著性差异(P<0.05或0.01)。HDAg以肝细胞核表达为主,其次是胞质表达,HBsAg以肝细胞浆表达为主,HDAg和HBsAg表达强度及阳性细胞分布呈一致性,两种抗原的表达程度与肝组织的炎症活动和病理损害相关(P<0.01)。HBcAg以以肝细胞核表达为主,阳性细胞主要呈单个细胞或点状分布,且HBcAg阳性细胞明显少于HDAg阳性细胞。HDAg表达强度与HBV DNA表达呈负相关(P<0.05)。结论 HDV感染会抑制HBV DNA复制或病毒抗原表达;在HDV致病机制中HDV的直接细胞毒性可能起主要作用,也有HBV的协同作用。
AIM To explore the relationship between expression of HDAg and HBsAg/HBcAg and HBV DNA in the liver tissues of the patients with hepatitis D (HD). METHODS HDAg, HBsAg, HBcAg and HBV DNA in paraffin-embedded liver tissue from 79 liver biopsy or autopsy samples from the patients with HD were studied with immunihistochemical double labelling techniques and hybridization in situ; and compared with the expression of HBsAg and HBcAg in liver tissues of the patients with hepatitis B (HB),RESULTS The detection rates for HBsAg, HBcAg and HBV DNA in liver specimens of HD were 81%, 71% and 27%, and were lower than that of hepatitis B(94%, 92% and 44%) (P<0.05 or 0.01). HDAg was mainly expressed in nuclei and cytoplasm of hepatocytes, most of positive cells were obviously aggregated in periportal region especially at the advancing edges of areas of piecemeal necrosis, moreover, strong expression of HDAg was shown in the ballooning degenerative hepatocytes and hepatocytes near piecemeal necrosis region. HBsAg was mainly expressed in cytoplasm of hepatocytes, and positive cell distribution was similar to HDAg positive cells. HBcAg was mainly expressed in nuclei of hepatocytes, positive cell distribution was alone or focal type (3-5 cells). Double labelling studies showed that both HDAg and HBsAg might be mostly expressed in the same hepatocy, and positive cells of HDAg and HBsAg were mostly found to locate in the same area of liver tissue, but HDAg coexisted seldomly with HBcAg, respectively. The expression degree and the positive cell distribution of HDAg and HBsAg were all obviously associated with the grades of pathological damage of liver tissue and necroinflammatory activity (P<0.01). CONCLUSION HDV infection can inhibit replication of HBV DNA or expression of HBV DNA antigen. Beside interactions between HBV and HDV, direct cytotoxicity of HDV may play an important pathogenetic role in pathogenetic mechanisms of HDV.
出处
《世界华人消化杂志》
CAS
2000年第1期31-34,共4页
World Chinese Journal of Digestology
关键词
丁型肝炎
丁型肝炎病毒
HDAG
hepatitis D
DNA, viral
hepatitis D virus
HBsAg
HBcAg
