摘要
目的 :探讨丁型肝炎病人肝组织中HDAg与HBsAg/HBcAg和HBVDNA表达及关系。 方法 :应用免疫组化双重染色和原位杂交 ,检测 79例丁型肝炎病人肝组织HDAg、HBsAg、HBcAg和HBVDNA表达 ,以 5 2例乙型肝炎作对照。结果 :丁型肝炎HBsAg、HBcAg检出率 ( 81%、71%)较乙型肝炎 ( 94%、92 %)低 (P<0 0 5或 0 0 1)。HDAg以肝细胞核表达为主 ,HBsAg以肝细胞浆表达为主 ,HDAg和HBsAg表达强度及阳性细胞分布呈一致性 ,且与肝组织的炎症活动和病理损害程度相关 (P <0 0 1)。HBcAg以肝细胞核表达为主 ,阳性细胞主要呈单个细胞或点状分布 ,且HBcAg阳性细胞明显少于HDAg阳性细胞。HDAg表达强度与HBVDNA表达呈负相关 (P <0 0 5 )。结论 :HDV感染会抑制HBVDNA复制或病毒抗原表达 ;在HDV致病机制中既有HDV的直接细胞毒性作用 ,也有HBV和HDV的协同作用。
Objective:To investigate the relationship among expressions of HDAg, HBs/HBcAg and HBV DNA in the liver in patients with hepatitis D Methods: HDAg, HBsAg, HBcAg and HBV DNA in paraffin embedded liver tissue from 79 patients with hepatitis D were detected with immunohistochemical double labeling and in situ hybridization Meanwhile, they were compared with the expressions of HBsAg and HBcAg in the liver tissues from patients with hepatitis B Results:The detecting rates of HBsAg, HBcAg and HBV DNA in liver specimens from patients with hepatitis D were significantly lower than those in patients with hepatitis B (P< 0 05 or 0 01) HDAg was mainly expressed in nuclei of hepatocytes Much of positive cells were obviously aggregated in periportal region, especially at the advancing edges of areas of piecemeal necrosis Moreover, strong expression of HDAg was seen in the bollooning degenerated hepatocytes and those near piecemeal necrosis region HBsAg was mainly expressed in cytoplasm of hepatocytes and positive cell distribution was similar to that of HDAg positive cells HBcAg was mainly expressed in nuclei of hepatocytes and positive cell distribution was alone for focal type Double labeling showed that both HDAg and HBsAg might be mostly expressed in the same hepatocytes and positive cells were mostly found to locate in the same area of the liver tissue Conclusion:HDV infection can inhibit replication of HBV or expression of HBV antigen Both the interaction between HBV and HDV and the direct cytotoxicity of HDV may play important roles in pathogenesis of hepatitis D
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第3期227-230,共4页
Journal of Third Military Medical University
