摘要
目的研究ＨＢｓＡｇ阴性肝病患者和自然人群中乙肝病毒（ＨＢＶ）携带者。方法采用ＤＮＡＰＣＲ直接法测定急、慢性肝炎，肝硬变和单项ＡＬＴ增高者共２３１例ＨＢｓＡｇ阴性患者中ＨＢＶＤＮＡ，２４０例ＨＢＶ标志阴性的自然人群对照。结果检出率在患者组和对照组分别为３３．７％（７８／２３１）和１１．５％（２８／２４０），Ｐ＜０．０１。单项ＡＬＴ增高组最高，达６６．７％（１０／１５），其他依次为慢肝组（ＣＨ）４１．９％（１３／３１）、肝硬变组（ＬＣ）３０．７％（５２／１６９）和急肝组１８．７％（３／１０）。单项ＡＬＴ增高组与ＬＣ、急肝组有显著差异（Ｐ＜０．０１）。
AIMS To study the HBV carriers in HBsAg negative patients with liver disease and natural people. METHODS HBV DNA were measured by DNA polymerase chain reaction serum direct assay(PCR) in 231 patients with liver pathology and 240 HBsAg negative natural people as controls. RESULTS The rates of positive of HBV DNA in patients and controls were 33.3%(78/231) and 11.5%(28/240), P<0.01. In simple ALT elevation persons it was the highest (66.7%, 10/15), and chronic hepatitis(CH), 41.9%(13/31), chronic persistent hepatitis(CPH), 38.4%(5/13), liver cirrhosis(LC), 30.7%(52/169) and acute 18.7%(3/16). There are significant difference between the simple ALT raised, LC and acute, P<0.01, in order. CONCLUSIONS There are HBV carriers in both HBsAg negative liver pathology patients and natural people.
关键词
聚合酶链反应
诊断
乙型肝炎
表面抗原
polymerase chain reaction hepatitis B/diagnosis carrier state/diagnosis hepatitis B virus/genetics DNA, viral/analysis hepatitis B surface antigens/analysis
