摘要
目的:比较第二代杂交捕获法(HCⅡ)与实时荧光聚合酶链反应技术(real-time PCR)在检测女性生殖道人乳头瘤病毒(HPV)高危亚型中的临床价值。方法:随机选取2011年1～6月我院妇产科行液基细胞薄层涂片技术进行低度鳞状上皮内病变(LSIL)检查,检查结果为未明确诊断意义的不典型鳞状上皮细胞(ASCUS)患者100例,分别采用real-time PCR与HCⅡ检测HPV高危亚型,比较两者的检测结果。对检测HPV阳性的样本或HPV阴性但液基细胞薄层涂片技术(LCT)≥LSIL的妇女采用阴道镜下活组织病理学检查,以病理结果作为验证两种HPV检测的参考标准。结果:共取病理68例。病理结果证实该人群中子宫颈上皮内瘤变(CIN)Ⅲ级5例,CINⅡ级11例,CINⅠ级19例,慢性宫颈炎和鳞状上皮化生33例。该100例患者HCⅡ和real-time PCR检测高危型HPV的阳性率分别为63.0%和71.0%,二者总符合率为82.3%;HCⅡ检测手段对高危宫颈病变患者进行高危HPV筛查的灵敏性、特异性、准确性、阳性预测值、阴性预测值、阳性似然比和阴性似然比分别为79.3%、88.2%、91.2%、10.1%、99.7%、8.9和0.327;real-time PCR以上各指标分别为100.0%、92.5%、86.7%、8.6%、100.0%、8.7和0。结论:Real-time PCR与HCⅡ检测宫颈上皮内瘤变患者高危型HPV有较好的相关性;但real-time PCR检测高危型HPV具有更好的敏感性和特异性。
Objective：To compare real-time polymerase chain reaction（real-time PCR） with hybrid capture Ⅱ（HC Ⅱ） in the detection of high-risk genotypes of human papillomavirus（HPV） in patients with cervical lesion.Methods：100 patients with cervical atypical squamous cells of undetetemined（TCT≥low grade squamous intraepithelial lesion,LSIL） who received treatment in our hospital department of obstetrics and gynecology were randomly selected from January 2011 to June 2011,cervical samples were detected by real-time PCR and HC Ⅱ to detect high-risk HPV subtypes,and the test results were compared.Results：68 cases of pathology were selected who were confirmed with cervical intraepithelial neoplasia（CIN）,and there were 5 cases were CIN Ⅲ,11 cease were CIN Ⅱ,19 cases were CIN I,33 cases were chronic cervicitis and squamous metaplasia.The positive rate of high-risk HPV types were tested by HC Ⅱ and multi-fluorescent real-time PCR were 63.0% and 71.0% respectively,the total coincidence rate of them was 82.3%.The sensitivity,specificity,accuracy,positive prevalue,negative prevalue,positive likelihood ratio and negative likelihood ratio of patients with cervical lesions detected by HC Ⅱ were 79.3%,88.2%,91.2%,10.1%,99.7%,8.9 and 0.327 respectively;Those of real-time PCR were 100.0%,92.5%,86.7%,8.6%,100.0%,8.7 and 0 respectively.Conclusion：There are good correlations between HC Ⅱ methods and multi-fluorescent real-time PCR methods in the detection of high-risk HPV type in patients with cervical intraepithelial neoplasia,however,real-time PCR detection of high-risk HPV has better sensitivity and specificity.
出处
《中国医药导报》
CAS
2011年第36期92-94,共3页
China Medical Herald
