摘要
目的：了解宫颈癌及上皮内瘤变人乳头瘤病毒（HPV）的感染率及其基因型的分布。方法：用PCR-RFLP法检测239例宫颈癌及上皮内瘤变患者HPV感染并进行分型。先用PGMY09／11共同引物扩增生殖道粘膜型HPV L1区的高度保守区，然后联合使用RsaⅠ、MseⅠ、PstⅠ和HaeⅢ4个限制性内切酶对阳性PCR产物进行酶切，利用不同的酶切片段鉴定HPV的基因型。结果：在239例宫颈癌及上皮内瘤变患者中共检出205例（85．8％）HPV感染，其中宫颈上皮内瘤变Ⅰ级（CINⅠ）、宫颈上皮内瘤变Ⅱ～Ⅲ级（CINⅡ-Ⅲ）和宫颈癌中HPV感染率分别是66．7％，89．9％和98．3％，差异有统计学意义（P〈0．001）。在宫颈癌及上皮内瘤变中共检出22型HPV，其中主要基因型及其感染率分别是HPV16（45．6％）、58（12．1％）和52（6．3％）。结论：宫颈癌及上皮内瘤变中HPV感染的基因型至少可达22型，其中以HPV16、58和52为最常见。
Objective： To investigate human papillomavirus （ HPV ） prevalence and HPV genotypes distribution in cervical carcinoma and intraepithelial neoplasia. Methods： PCRRFLP assay was used to detect HPV genotypes in 239 cases of women with cervical carcinoma and intrapithelial neoplasia. Consensus primer PGMY09/11 was used to amplify genital HPV highly conserved L1 region,and restriction enzymes Rsa Ⅰ ,Mse Ⅰ ,Pst Ⅰ and HaeⅢ were used to digest PCR products and identify HPV genotypes. Results： HPV infection was detected in 205 of 239 cases（ 85.8% ） with cervical carcinoma and intraepithelial neoplasia. HPV infection in cervical intraepithelial neoplasia grade Ⅰ （CIN Ⅰ ）, cervical intraepithelial neoplasia grade Ⅱ-Ⅲ （ CIN Ⅱ-Ⅲ ） and cervical cancer were 66. 7%, 89. 9% and 98.3% respectively, and there were statistical significance （P 〈0. 001 ）. Twenty-two types of HPV genotypes were identified in cervical carcinoma and intraepithelial neoplasia, and more common genotypes were HPV16（45.6% ） ,58 （ 12. 1% ） and 52 （6. 3% ）. Conclusion： Twenty-two HPV genotypes at least are involved in the HPV infection in cervical carcinoma and intraepithelial neoplasia. Among them, HPV16,58 and 52 are the most common.
出处
《现代妇产科进展》
CSCD
北大核心
2006年第1期36-38,共3页
Progress in Obstetrics and Gynecology
基金
浙江省科技厅科研基金资助课题(2004C33053)
关键词
乳头状瘤病毒
人
基因型
宫颈肿瘤
宫颈上皮内瘤变
Papillomavirus, human
Genotype
Cervix neoplasms
Cervical intraepithelial neoplasia
